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Cross-reactivity of highly purified okadaic acid (OA), synthetic, spiroketal East sphere of OA and ciguatoxin.
- Source :
-
Journal of clinical laboratory analysis [J Clin Lab Anal] 1992; Vol. 6 (1), pp. 54-8. - Publication Year :
- 1992
-
Abstract
- This study presents data from the cross-reactivity analysis of purified ciguatoxin (CTX), okadaic acid (OA), and the East sphere or Fragment B-C of OA with their homologous antibodies, monoclonal antibodies to ciguatoxin (MAb-CTX) and okadaic acid (MAb-OA). The test system used was the stick enzyme immunoassay. MAb-CTX gave peak titers of 1.5ng, 10ng and 50ng respectively for CTX, East sphere and OA. Competitive inhibition analysis showed that 4ng purified CTX blocked completely MAb-CTX reaction with crude CTX, OA and East sphere of OA blocked at similar concentrations (approximately 50ng). The activity with MAb-OA in the homologous system with OA and East sphere was insignificant. This may be attributable to the improper concentrations used. The cross-reactivity between MAb-CTX with OA and its Fragment B-C may cause difficulty in the test system in its application to assess toxic fish due to ciguatoxin.
Details
- Language :
- English
- ISSN :
- 0887-8013
- Volume :
- 6
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of clinical laboratory analysis
- Publication Type :
- Academic Journal
- Accession number :
- 1542084
- Full Text :
- https://doi.org/10.1002/jcla.1860060111