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Serological identification of TROP2 by recombinant cDNA expression cloning using sera of patients with esophageal squamous cell carcinoma.
- Source :
-
International journal of cancer [Int J Cancer] 2004 Dec 20; Vol. 112 (6), pp. 1029-35. - Publication Year :
- 2004
-
Abstract
- We applied serological analysis of recombinant cDNA expression libraries (SEREX) to cases of esophageal squamous cell carcinoma (SCC) to identify tumor antigens. One of the clones identified was TROP2, which is known as calcium signal transducer. To evaluate the clinical significance of serum anti-TROP2 antibodies (s-TROP2-Abs) in patients with esophageal SCC, the presence of s-TROP2-Abs was analyzed by Western blotting using bacterially expressed TROP2 protein. We found that 23 of 75 (31%) patients were positive for s-TROP2-Abs. Positivity in terms of s-TROP2-Abs showed a significant association with tumor size but not with other clinicopathological features. The protein expression levels of TROP2 were much higher in esophageal SCC cell lines as compared to those in normal esophageal mucosa and its immortalized cells although the mRNA expression levels were not necessarily elevated in malignant cell lines and tissues. Immunohistochemical studies showed that the expression of TROP2 protein in esophageal SCC specimens was noticeably higher than that found in mild hyperplasia of esophageal mucosae. Thus, s-TROP2-Abs seemed useful in the diagnosis of SCC and may be a candidate for serum tumor markers.<br /> ((c) 2004 Wiley-Liss, Inc.)
- Subjects :
- Aged
Antigens, Neoplasm immunology
Biomarkers, Tumor immunology
Blotting, Western
Cell Adhesion Molecules immunology
Cell Line, Tumor
Cloning, Molecular methods
DNA, Complementary analysis
DNA, Neoplasm analysis
Epithelial Cell Adhesion Molecule
Female
Gene Expression Regulation, Neoplastic
Humans
Immunohistochemistry
Male
Middle Aged
Recombinant Proteins analysis
Antigens, Neoplasm analysis
Biomarkers, Tumor analysis
Carcinoma, Squamous Cell chemistry
Carcinoma, Squamous Cell diagnosis
Cell Adhesion Molecules analysis
Esophageal Neoplasms chemistry
Esophageal Neoplasms diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 0020-7136
- Volume :
- 112
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- International journal of cancer
- Publication Type :
- Academic Journal
- Accession number :
- 15386348
- Full Text :
- https://doi.org/10.1002/ijc.20517