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Differential expression of integrin subunits in DU-145/AR prostate cancer cells.
- Source :
-
Oncology reports [Oncol Rep] 2004 Oct; Vol. 12 (4), pp. 837-41. - Publication Year :
- 2004
-
Abstract
- We have established a clonal DU-145 prostate cancer cell line (DU-145/AR) stably transfected with androgen receptor cDNA. We investigated the expression of integrin subunits, adhesion to extracellular matrices, the invasion of DU-145/AR prostate cancer cells. The expression of various integrin subunits and adhesion to various extracellular matrices in DU-145, DU-145/Neo and DU-145/AR cells were examined. The haptoinvasion and the haptotactic migration of these cells were investigated using a Transwell cell culture chamber assay. DU-145/AR cells exhibited lower expression of alpha6 and beta4 integrin subunits and higher expression of alpha2 and alpha5 than DU-145 cells. DU-145/AR cells showed significantly lower adhesion to fibronectin, laminin-1 and laminin-5 than DU-145/ Neo cells, whereas DU-145/AR cells showed higher adhesion to type I and type IV collagen. Haptoinvasion of DU-145/AR cells into Matrigel/fibronectin-coated filter was significantly reduced as compared with DU-145/Neo or DU-145 cells, but there was no significant difference between DU-145/AR and control cells in the haptotactic migration to fibronectin. Dihydrotestosterone (DHT) inhibited the invasive ability of DU-145/AR cells. These results indicate that androgen receptor may play a role in the regulation of adhesion to the extracellular matrices and invasion of prostate cancer cells through influencing the expression of specific integrin subunits.
- Subjects :
- Androgens pharmacology
Cell Adhesion Molecules metabolism
Cell Line, Tumor
Collagen Type I metabolism
Collagen Type IV metabolism
DNA, Complementary genetics
DNA, Complementary metabolism
Dihydrotestosterone pharmacology
Extracellular Matrix
Fibronectins metabolism
Humans
Integrin alpha2 metabolism
Integrin alpha5 metabolism
Integrin alpha6 metabolism
Integrin beta4 metabolism
Laminin metabolism
Male
Protein Subunits
Receptors, Androgen genetics
Transfection
Kalinin
Cell Adhesion
Cell Movement
Neoplasm Invasiveness pathology
Prostatic Neoplasms metabolism
Receptors, Androgen metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1021-335X
- Volume :
- 12
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Oncology reports
- Publication Type :
- Academic Journal
- Accession number :
- 15375509