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Increased presence of matrix metalloproteinases 2 and 9 in short- and long-term experimental herpes simplex virus encephalitis.
- Source :
-
Neuroscience letters [Neurosci Lett] 2004 Sep 30; Vol. 368 (3), pp. 274-8. - Publication Year :
- 2004
-
Abstract
- Herpes simplex virus encephalitis (HSVE) causes elevated morbidity and mortality despite antiviral treatment. Virus-independent mechanisms may perpetuate brain damage. Matrix metalloproteinases (MMPs) target extracellular matrix components. This study describes the protein and mRNA expression of MMP2 and MMP9 in experimental HSVE in the short and long term. Ten SJL-NBOM mice were infected with neurovirulent HSV-1 and compared with nine controls. The presence of MMP2 and MMP9 in brain tissue was analyzed with sodium-dodecyl-sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) gelatin zymography and mRNA expression of MMP2 and MMP9 with quantitative real-time PCR at days 7, 21 and 180 post-inoculation. Infected animals had a significantly elevated gelatinolytic activity of MMP2 at all time points, and of MMP9 at days 21 and 180. Increased presence of MMP2 and MMP9 in chronic HSVE may contribute to ongoing damage. Inhibition of MMP2 and MMP9 might be a suitable target for therapeutic intervention.
- Subjects :
- Animals
Brain enzymology
Brain virology
Disease Models, Animal
Encephalitis, Herpes Simplex genetics
Encephalitis, Herpes Simplex pathology
Female
Herpesvirus 1, Human genetics
Matrix Metalloproteinase 2 genetics
Matrix Metalloproteinase 9 genetics
Mice
RNA, Messenger biosynthesis
RNA, Messenger genetics
Time Factors
Encephalitis, Herpes Simplex enzymology
Herpesvirus 1, Human enzymology
Matrix Metalloproteinase 2 biosynthesis
Matrix Metalloproteinase 9 biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 0304-3940
- Volume :
- 368
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Neuroscience letters
- Publication Type :
- Academic Journal
- Accession number :
- 15364410
- Full Text :
- https://doi.org/10.1016/j.neulet.2004.06.064