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The pepper transcription factor CaPF1 confers pathogen and freezing tolerance in Arabidopsis.
- Source :
-
Plant physiology [Plant Physiol] 2004 Sep; Vol. 136 (1), pp. 2862-74. Date of Electronic Publication: 2004 Sep 03. - Publication Year :
- 2004
-
Abstract
- An ERF/AP2-type transcription factor (CaPF1) was isolated by differential-display reverse transcription-PCR, following inoculation of the soybean pustule pathogen Xanthomonas axonopodis pv glycines 8ra, which induces hypersensitive response in pepper (Capsicum annuum) leaves. CaPF1 mRNA was induced under conditions of biotic and abiotic stress. Higher levels of CaPF1 transcripts were observed in disease-resistant tissue compared with susceptible tissue. CaPF1 expression was additionally induced using various treatment regimes, including ethephon, methyl jasmonate, and cold stress. To determine the role of CaPF1 in plants, transgenic Arabidopsis and tobacco (Nicotiana tabacum) plants expressing higher levels of CaPF1 were generated. Gene expression analyses of transgenic Arabidopsis and tobacco revealed that the CaPF1 level in transgenic plants affects expression of genes that contain either a GCC or a CRT/DRE box in their promoter regions. Furthermore, transgenic Arabidopsis plants expressing CaPF1 displayed tolerance against freezing temperatures and enhanced resistance to Pseudomonas syringae pv tomato DC3000. Disease tolerance was additionally observed in CaPF1 transgenic tobacco plants. The results collectively indicate that CaPF1 is an ERF/AP2 transcription factor in hot pepper plants that may play dual roles in response to biotic and abiotic stress in plants.
- Subjects :
- Amino Acid Sequence
Arabidopsis microbiology
Base Sequence
Binding Sites genetics
DNA, Plant genetics
DNA, Plant metabolism
Freezing
Gene Expression
Genes, Plant
Molecular Sequence Data
Phylogeny
Plants, Genetically Modified
Pseudomonas syringae pathogenicity
RNA, Messenger genetics
RNA, Messenger metabolism
RNA, Plant genetics
RNA, Plant metabolism
Recombinant Proteins genetics
Recombinant Proteins metabolism
Sequence Homology, Amino Acid
Tissue Distribution
Nicotiana genetics
Nicotiana microbiology
Nicotiana physiology
Arabidopsis genetics
Arabidopsis physiology
Capsicum genetics
Capsicum physiology
Transcription Factors genetics
Transcription Factors physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0032-0889
- Volume :
- 136
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Plant physiology
- Publication Type :
- Academic Journal
- Accession number :
- 15347795
- Full Text :
- https://doi.org/10.1104/pp.104.042903