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[Human anti-HCMV neutralizing Fab antibody generated by phage display library].
- Source :
-
Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology [Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi] 2003 Sep; Vol. 17 (3), pp. 247-50. - Publication Year :
- 2003
-
Abstract
- Background: Cloning recombinant human Fab fragment against HCMV for the purpose of prophylaxis and control of HCMV infection.<br />Methods: The authors constructed a HCMV phage display library with 2 x 10(6) clones, then used purified HCMV viral lysates to pan the library, then screened by ELISA.<br />Results: Three clones showed positive responses in ELISA, they also showed high specificity in IFA, two of them could neutralize HCMV in neutralizing assays.<br />Conclusion: The specific binding of Fab antibodies to HCMV was demonstrated by ELISA, IFA and neutralizing activities. These results provide us the basis for further research of neutralizing recombinant human whole IgG molecule.
- Subjects :
- Antibodies, Viral genetics
Cytomegalovirus genetics
Cytomegalovirus Infections immunology
Cytomegalovirus Infections virology
Escherichia coli genetics
Escherichia coli metabolism
Gene Expression
Humans
Immunoglobulin Fab Fragments genetics
Neutralization Tests
Antibodies, Viral immunology
Cytomegalovirus immunology
Immunoglobulin Fab Fragments immunology
Peptide Library
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1003-9279
- Volume :
- 17
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology
- Publication Type :
- Academic Journal
- Accession number :
- 15340569