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Synthesis of DNA using a new two-step cycle.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2005; Vol. 288, pp. 1-16. - Publication Year :
- 2005
-
Abstract
- For the first time a new, two-step method is described for synthesizing deoxyribonucleic acid. The approach uses 5'-carbonate protected 2'-deoxynucleoside-3'-phosphoramidites as synthons and a peroxy anion buffer that removes the carbonate protecting group and oxidizes the internucleotide linkage. Following synthesis via this two-step cycle, oligomers are isolated by standard procedures.
- Subjects :
- Amides chemistry
Chromatography, High Pressure Liquid
Electrophoresis, Polyacrylamide Gel
Indicators and Reagents chemistry
Magnetic Resonance Spectroscopy
Oxidation-Reduction
Phosphoric Acids chemistry
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
DNA chemical synthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1064-3745
- Volume :
- 288
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 15333894
- Full Text :
- https://doi.org/10.1385/1-59259-823-4:001