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Metallic dental material biocompatibility in osteoblastlike cells: correlation with metal ion release.
Metallic dental material biocompatibility in osteoblastlike cells: correlation with metal ion release.
- Source :
-
Biological trace element research [Biol Trace Elem Res] 2004 Aug; Vol. 100 (2), pp. 151-68. - Publication Year :
- 2004
-
Abstract
- Ions released from metallic dental materials used in orthodontic appliances could induce undesirable effects on cells and tissues. This study evaluates the biocompatibility of two of the most labile components of metallic dental alloys on osteoblastlike cells. The influence of protein and ions on metal dissolution properties is also investigated using different electrolyte solutions. Morphological alterations, cell growth, and differentiation of osteoblasts were assessed after exposure to pure metals (Ag, Cu, Pd, Au) and Ni-Ti alloy and correlated with the kinetics of elements released into the culture media. Results showed that Cu and Ag were the most cytotoxic elements and the other metals were biocompatible with the osteoblasts. The parameters of biocompatibility were correlated with the levels of ions detected into the culture media. Metal ions induced cell death through early mitosis arrest, apoptotic phenomena, and necrotic processes. Voltammograms showed that anions and proteins interfered in the corrosion process. Fetal bovine serum (FBS) strongly affected the electrochemical process, decreasing the oxidation rate of the metals. In conclusion, copper and silver ions showed a time-dependent low biocompatibility, which correlated with the concentration of released ions. The dissolution of the metallic materials was dependent on the composition of the simulated biological media.
- Subjects :
- Animals
Cell Differentiation
Cell Line, Tumor
Cell Proliferation drug effects
Copper chemistry
Copper metabolism
Culture Media
Dental Alloys
Dose-Response Relationship, Drug
Electrochemistry
Electrolytes
Gold metabolism
Humans
Ions
Kinetics
Materials Testing
Metals
Nickel blood
Orthodontics
Osteoblasts drug effects
Osteosarcoma blood
Oxygen metabolism
Palladium metabolism
Rats
Silver metabolism
Spectrophotometry, Atomic
Time Factors
Titanium blood
Biocompatible Materials
Osteoblasts metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0163-4984
- Volume :
- 100
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Biological trace element research
- Publication Type :
- Academic Journal
- Accession number :
- 15326364
- Full Text :
- https://doi.org/10.1385/bter:100:2:151