[Gene expression profile in early differentiation of K562 cells induced by matrine].

Authors :: Zhang Y
Ma LD
He YJ
Jiang JK
Source :: Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi [Zhonghua Xue Ye Xue Za Zhi] 2004 Jun; Vol. 25 (6), pp. 342-5.
Publication Year :: 2004
Abstract: Objective: To compare gene expression profile in K562 cells induced by matrine, so as to screen for differentiation related genes.<br />Methods: K562 cells were exposed to 0.1 mg/ml matrine for 3 hours, and gene expression profiles in matrine-treated and non-treated cells were studied by cDNA microarray.<br />Results: From 8465 screened genes, 30 differentially expressed genes were found. Among them 18 showed decreased expression including oncogene and proto-oncogene, signal transduction gene, DNA binding and transcription gene etc, 12 showed increased expression including cell receptor gene, immune-associated gene, metabolism-associated gene etc in matrine-treated K562 cells as compared with that in non-treated cell.<br />Conclusion: The genes, that are closely associated with differentiation of can-cer cell, could be the potential targets for cancer treatment.

Subjects :: Cell Differentiation genetics
Gene Expression Profiling
Gene Expression Regulation, Neoplastic drug effects
Humans
K562 Cells cytology
K562 Cells drug effects
Oligonucleotide Array Sequence Analysis
Proto-Oncogene Mas
Matrines
Alkaloids pharmacology
Cell Differentiation drug effects
K562 Cells metabolism
Quinolizines pharmacology

Tools