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Molecular identification, localization and function of platelet-type 12-lipoxygenase in human melanoma progression, under experimental and clinical conditions.

Authors :
Rásó E
Döme B
Somlai B
Zacharek A
Hagmann W
Honn KV
Tímár J
Source :
Melanoma research [Melanoma Res] 2004 Aug; Vol. 14 (4), pp. 245-50.
Publication Year :
2004

Abstract

As previous studies suggested the expression of a 12-LOX enzyme in murine and human melanoma cell lines, the primary aim of this project was to genetically identify the 12-LOX enzyme (platelet-, leukocyte- or epithelial form). By using reverse transcriptase-polymerase chain reaction, sequencing and various immunological techniques we have demonstrated conclusively the expression of the platelet-type 12-LOX in human melanoma cells of different origin, in their transplanted xenografts and in fresh human skin tumors. Furthermore, we found that p12-LOX is able to provide a survival signal for melanoma cells since inhibition of the enzyme by general LOX or selective 12-LOX inhibitors induced apoptosis in vitro. p12-LOX of human melanoma has been shown to be involved in the control of the metastatic phenotype, since we have detected the upregulation of the 12-LOX protein expression in spontaneously metastasizing xenografts and in thick human skin tumors (> 3.0 mm) characterized by high risk for the development of metastasis. Co-expression of two megakaryocytic genes, p12-LOX and alphaIIb integrin chains, was found to be a frequent phenomenon in human melanoma (approximately 70%) suggesting a common regulatory defect in this tumor.<br /> (Copyright 2004 Lippincott Williams & Wilkins)

Details

Language :
English
ISSN :
0960-8931
Volume :
14
Issue :
4
Database :
MEDLINE
Journal :
Melanoma research
Publication Type :
Academic Journal
Accession number :
15305153
Full Text :
https://doi.org/10.1097/01.cmr.0000138479.00897.d8