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Classification of hyper-variable Corynebacterium glutamicum surface-layer proteins by sequence analyses and atomic force microscopy.
- Source :
-
Journal of biotechnology [J Biotechnol] 2004 Aug 26; Vol. 112 (1-2), pp. 177-93. - Publication Year :
- 2004
-
Abstract
- The structural S-layer proteins of 28 different Corynebacterium glutamicum isolates have been analyzed systematically. Treatment of whole C. glutamicum cells with detergents resulted in the isolation of S-layer proteins with different apparent molecular masses, ranging in size from 55 to 66 kDa. The S-layer genes analyzed were characterized by coding regions ranging from 1,473 to 1,533 nucleotides coding for S-layer proteins with a size of 490-510 amino acids. Using PCR techniques, the corresponding S-layer genes of the 28 C. glutamicum isolates were all cloned and sequenced. The deduced amino acid sequences of the S-layer proteins showed identities between 69 and 98% and could be grouped into five phylogenetic classes. Furthermore, sequence analyses indicated that the S-layer proteins of the analyzed C. glutamicum isolates exhibit a mosaic structure of highly conserved and highly variable regions. Several conserved regions were assumed to play a key role in the formation of the C. glutamicum S-layers. Especially the N-terminal signal peptides and the C-terminal anchor sequences of the S-layer proteins showed a nearly perfect amino acid sequence conservation. Analyses by atomic force microscopy revealed a committed hexagonal structure. Morphological diversity of the C. glutamicum S-layers was observed in a class-specific unit cell dimension (ranging from 15.2 to 17.4 nm), which correlates with the sequence similarity-based classification. It could be demonstrated that differences in the primary structure of the S-layer proteins were reflected by the S-layer morphology.
- Subjects :
- Amino Acid Sequence
Bacterial Proteins ultrastructure
Membrane Glycoproteins ultrastructure
Molecular Sequence Data
Polymerase Chain Reaction
Protein Conformation
Sequence Alignment methods
Sequence Homology, Amino Acid
Bacterial Proteins chemistry
Bacterial Proteins classification
Corynebacterium glutamicum metabolism
Membrane Glycoproteins chemistry
Membrane Glycoproteins classification
Microscopy, Atomic Force methods
Sequence Analysis, Protein methods
Subjects
Details
- Language :
- English
- ISSN :
- 0168-1656
- Volume :
- 112
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Journal of biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 15288952
- Full Text :
- https://doi.org/10.1016/j.jbiotec.2004.03.020