Back to Search
Start Over
High nucleotide and amino acid sequence similarities in tumour necrosis factor-alpha amongst Indian buffalo (Bubalus bubalis), Indian cattle (Bos indicus) and other ruminants.
- Source :
-
European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics [Eur J Immunogenet] 2004 Aug; Vol. 31 (4), pp. 189-93. - Publication Year :
- 2004
-
Abstract
- Tumour necrosis factor-alpha (TNF-alpha) mRNA from Indian water buffalo (Bubalus bubalis) and Indian cattle (Bos indicus) was reverse transcribed and amplified using reverse transcriptase-polymerase chain reaction (RT-PCR). The nucleotide sequences of cDNAs were determined after cloning into pGEM-T-Easy vector (Promega, Madison, WI) and compared with reported nucleotide sequences of TNF-alpha cDNA from other species. The nucleotide sequences of TNF-alpha from Indian cattle revealed significantly high similarities at nucleotide (99.2%) and amino acid (100%) levels with those of cattle (Bos taurus; Zebu). The sequences from buffalo had 98.4% nucleotide and 99.1% amino acid similarities with Indian cattle, indicating functional cross-reactivity. One amino acid deletion at position 63 and one substitution (A-->P) at position 64 were observed in buffalo compared with Indian cattle. The amino acid deletion at position 63 was predicted due to differences in pre-mRNA splicing.
Details
- Language :
- English
- ISSN :
- 0960-7420
- Volume :
- 31
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics
- Publication Type :
- Academic Journal
- Accession number :
- 15265025
- Full Text :
- https://doi.org/10.1111/j.1365-2370.2004.00471.x