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Characterization of mesentericin ST99, a bacteriocin produced by Leuconostoc mesenteroides subsp. dextranicum ST99 isolated from boza.
- Source :
-
Journal of industrial microbiology & biotechnology [J Ind Microbiol Biotechnol] 2004 Aug; Vol. 31 (7), pp. 323-9. Date of Electronic Publication: 2004 Jul 14. - Publication Year :
- 2004
-
Abstract
- Lactic acid bacteria isolated from Boza, a cereal-fermented beverage from Belogratchik, Bulgaria, were screened for the production of bacteriocins. With the first screening, 13 of the 52 isolates inhibited the growth of Listeria innocua and Lactobacillus plantarum. The cell-free supernatant of one of these strains, classified as Leuconostoc mesenteroides subsp. dextranicum ST99, inhibited the growth of Bacillus subtilis, Enterococcus faecalis, several Lactobacillus spp., Lactococcus lactis subsp. cremoris, Listeria innocua, Listeria monocytogenes, Pediococcus pentosaceus, Staphylococcus aureus and Streptococcus thermophilus. Clostridium spp., Carnobacterium spp., L. mesenteroides and Gram-negative bacteria were not inhibited. Maximum antimicrobial activity, i.e. 6,400 arbitrary units (AU)/ml, was recorded in MRS broth after 24 h at 30 degrees C. Incubation in the presence of protease IV and pronase E resulted in loss of antimicrobial activity, confirming that growth inhibition was caused by a bacteriocin, designated here as mesentericin ST99. No loss in activity was recorded after treatment with alpha-amylase, SDS, Tween 20, Tween 80, urea, Triton X-100, N-laurylsarcosin, EDTA and phenylmethylsulfonylfluoride. Mesentericin ST99 remained active after 30 min at 121 degrees C and after 2 h of incubation at pH 2 to 12. Metabolically active cells of L. innocua treated with mesentericin ST99 did not undergo lysis. Mesentericin ST99 did not adhere to the cell surface of strain ST99. Precipitation with ammonium sulfate (70% saturation), followed by Sep-Pack C18 chromatography and reverse-phase HPLC on a C18 Nucleosil column yielded one antimicrobial peptide.
- Subjects :
- Anti-Bacterial Agents biosynthesis
Anti-Bacterial Agents metabolism
Anti-Bacterial Agents pharmacology
Bacillus subtilis drug effects
Bacillus subtilis growth & development
Bacteriocins chemistry
Bacteriocins isolation & purification
Chelating Agents pharmacology
Clostridium drug effects
Clostridium growth & development
Culture Media chemistry
Gram-Negative Bacteria drug effects
Gram-Negative Bacteria growth & development
Hydrogen-Ion Concentration
Lactobacillus drug effects
Lactobacillus growth & development
Listeria drug effects
Listeria growth & development
Peptide Hydrolases metabolism
Pronase metabolism
Protease Inhibitors pharmacology
Staphylococcus aureus drug effects
Staphylococcus aureus growth & development
Streptococcaceae drug effects
Streptococcaceae growth & development
Surface-Active Agents pharmacology
Temperature
alpha-Amylases metabolism
Bacteriocins biosynthesis
Bacteriocins pharmacology
Leuconostoc metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1367-5435
- Volume :
- 31
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Journal of industrial microbiology & biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 15252717
- Full Text :
- https://doi.org/10.1007/s10295-004-0153-6