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Inhibitory effects of Cimicifuga heracleifolia extract on glutamate formation and glutamate dehydrogenase activity in cultured islets.
- Source :
-
Molecules and cells [Mol Cells] 2004 Jun 30; Vol. 17 (3), pp. 509-14. - Publication Year :
- 2004
-
Abstract
- Hyperinsulinism-hyperammonemia syndrome is due either to hyperactivity of GDH or impaired inhibition of GDH by GTP. We have investigated the effect of Cimicifuga heracleifolia extract on the activities of glutamate dehydrogenase (GDH) in cultured rat islets. When the extract was present in the culture medium for 24 h prior to cell harvest, the Vmax of GDH was decreased by 45% with no significant change in Km. In addition, the concentration of alpha-ketoglutarate increased by approximately 39%, and glutamate decreased by 48%. Perfusion of islets with C. heracleifolia extract reduced insulin release by up to 47%. Although the relation between GDH activity and insulin release remains to be clarified, our results suggest that C. heracleifolia extract regulates insulin release by altering GDH activity in primary cultured islets and that this natural compound may be used to modulate GDH activity in patients with hyperinsulinism-hyperammonemia syndrome.
- Subjects :
- Animals
Enzyme Activation drug effects
Enzyme Inhibitors toxicity
Glutamate Dehydrogenase antagonists & inhibitors
Insulin metabolism
Insulin Secretion
Islets of Langerhans drug effects
Mitochondria metabolism
Plant Extracts toxicity
Rats
Rats, Sprague-Dawley
Cimicifuga toxicity
Glutamate Dehydrogenase metabolism
Glutamic Acid metabolism
Islets of Langerhans enzymology
Ketoglutaric Acids metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1016-8478
- Volume :
- 17
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Molecules and cells
- Publication Type :
- Academic Journal
- Accession number :
- 15232227