Poly(dimethylsiloxane) microchip capillary electrophoresis with electrochemical detection for rapid measurement of acetaminophen and its hydrolysate.

Authors :: He FY
Liu AL
Xia XH
Source :: Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2004 Aug; Vol. 379 (7-8), pp. 1062-7. Date of Electronic Publication: 2004 Jun 18.
Publication Year :: 2004
Abstract: Poly(dimethylsiloxane) microchip capillary electrophoresis with amperometric detection has been used for rapid separation and determination of acetaminophen and its hydrolysate, i.e. p-aminophenol. A Pt ultramicroelectrode with a diameter of 10 microm positioned at the outlet of the separation channel was used as a working electrode for amperometric detection. Factors influencing separation and detection were investigated and optimized. Results show that acetaminophen and p-aminophenol can be well separated within 35 s with RSD-1 (approximately 0.1 fmol) at S/ N=3. This method has been successfully applied to the detection of traces of p-aminophenol in paracetamol tablets.

Subjects :: Acetaminophen chemistry
Electrochemistry
Electrophoresis, Capillary instrumentation
Electrophoresis, Microchip instrumentation
Hydrolysis
Sensitivity and Specificity
Time Factors
Acetaminophen analysis
Aminophenols analysis
Dimethylpolysiloxanes chemistry
Electrophoresis, Capillary methods
Electrophoresis, Microchip methods
Polymers chemistry

Full Text Access

Tools