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Transmission of HIV-1 by primary human uterine epithelial cells and stromal fibroblasts.
- Source :
-
The Journal of infectious diseases [J Infect Dis] 2004 Jul 15; Vol. 190 (2), pp. 236-45. Date of Electronic Publication: 2004 Jun 11. - Publication Year :
- 2004
-
Abstract
- Women can become infected with human immunodeficiency virus type 1 (HIV-1) after the heterosexual transmission of virus from an infected male partner. To understand the events that result in transmission of HIV-1 across the female reproductive tract, we characterized the life-cycle events of HIV-1 in primary cultures of human uterine epithelial cells and stromal fibroblasts. Epithelial cells and stromal fibroblasts released virus particles after exposure to either X4- or R5-tropic strains of HIV-1. Virus released by these cells was able to infect CD4(+) T cells. When exposed to an X4-tropic strain of HIV-1, these cells supported HIV-1 reverse transcription, integration, and viral DNA transcription. When exposed to an R5-tropic strain, however, these cells released unmodified virus. These data suggest that uterine cells are targets for productive infection with X4-tropic strains and release unmodified R5-tropic viruses that would then be able to infect submucosal target cells, including T cells and macrophages.
- Subjects :
- CD4-Positive T-Lymphocytes virology
Cells, Cultured
Female
HIV Core Protein p24 analysis
HIV-1 physiology
Humans
Receptors, CCR5 metabolism
Receptors, CXCR4 biosynthesis
Receptors, CXCR4 metabolism
Receptors, HIV metabolism
Stromal Cells virology
Transcription, Genetic
Virus Integration
Virus Replication
Epithelial Cells virology
Fibroblasts virology
HIV Infections transmission
HIV-1 pathogenicity
Uterus cytology
Uterus virology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1899
- Volume :
- 190
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- The Journal of infectious diseases
- Publication Type :
- Academic Journal
- Accession number :
- 15216456
- Full Text :
- https://doi.org/10.1086/421910