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Nuclear factor-kappaB regulates inflammatory cell apoptosis and phagocytosis in rat carrageenin-sponge implant model.
- Source :
-
The American journal of pathology [Am J Pathol] 2004 Jul; Vol. 165 (1), pp. 115-26. - Publication Year :
- 2004
-
Abstract
- In the present study we investigated whether apoptosis and phagocytosis are regulated by nuclear factor (NF)-kappaB in a model of chronic inflammation. The subcutaneous implant of lambda-carrageenin-soaked sponges elicited an inflammatory response, characterized by a time-related increase of leukocyte infiltration into the sponge and tissue formation, which was inhibited by simultaneous injection of wild-type oligodeoxynucleotide decoy to NF-kappaB. Molecular and morphological analysis performed on infiltrated cells demonstrated: 1) an inhibition of NF-kappaB/DNA binding activity; 2) an increase of polymorphonuclear leukocyte apoptosis correlated either to an increase of p53 or Bax and decrease of Bcl-2 protein expression; and 3) an increase of phagocytosis of apoptotic polymorphonuclear leukocytes by macrophages associated with an increase of transforming growth factor-beta1 and decrease of tumor necrosis factor-alpha as well as nitrite/nitrate production. Our results, showing that blockade of NF-kappaB by oligodeoxynucleotide decoy increases inflammatory cell apoptosis and phagocytosis, may contribute to lead to new insights into the mechanisms governing the inflammatory process.
- Subjects :
- Animals
Blotting, Western
Cell Movement
Disease Models, Animal
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Immunohistochemistry
Inflammation drug therapy
Inflammation metabolism
Male
Neutrophils drug effects
Neutrophils ultrastructure
Nitrates analysis
Nitrites analysis
Oligonucleotides therapeutic use
Rats
Rats, Wistar
Time Factors
Transcription Factors therapeutic use
Transforming Growth Factor beta analysis
Transforming Growth Factor beta1
Apoptosis drug effects
Carrageenan toxicity
Inflammation chemically induced
NF-kappa B metabolism
Neutrophils metabolism
Phagocytosis drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0002-9440
- Volume :
- 165
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- The American journal of pathology
- Publication Type :
- Academic Journal
- Accession number :
- 15215167
- Full Text :
- https://doi.org/10.1016/s0002-9440(10)63280-4