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[Cloning and expression of Fab gene of mAb HAb18 against human hepatocellular carcinoma].
- Source :
-
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology [Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi] 2003 Nov; Vol. 19 (6), pp. 570-3. - Publication Year :
- 2003
-
Abstract
- Aim: To clone Fab gene of mAb HAb18 against human hepatocellular carcinoma and express it in E.coli.<br />Methods: The cDNAs of kappa chain and Fd of mAb HAb18 were amplified by RT-PCR and cloned into prokaryotic expression vector pComb3 which was transfected into competent E.coli to express Fab by IPTG induction. The specificity of the expressed Fab was tested by ELISA and immunofluorescence staining.<br />Results: The Fab gene of mAb HAb18 was successfully amplified and expressed in E.coli. The results of competitive ELISA and immunofluorescence staining showed that the expressed Fab had specific antigen-binding activity.<br />Conclusion: HAb18 Fab was prepared successfully, which lays the foundation for its further application to diagnosis and therapy of human hepatocellular carcinoma.
- Subjects :
- Antibodies, Monoclonal immunology
Base Sequence
Cloning, Molecular
Enzyme-Linked Immunosorbent Assay
Escherichia coli genetics
Fluorescent Antibody Technique
Humans
Immunoglobulin Fab Fragments immunology
Molecular Sequence Data
Reverse Transcriptase Polymerase Chain Reaction
Antibodies, Monoclonal genetics
Carcinoma, Hepatocellular immunology
Immunoglobulin Fab Fragments genetics
Liver Neoplasms immunology
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1007-8738
- Volume :
- 19
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
- Publication Type :
- Academic Journal
- Accession number :
- 15182653