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Expression and functional effects of Eph receptor tyrosine kinase A family members on Langerhans like dendritic cells.
- Source :
-
BMC immunology [BMC Immunol] 2004 Jun 03; Vol. 5, pp. 9. Date of Electronic Publication: 2004 Jun 03. - Publication Year :
- 2004
-
Abstract
- Background: The Eph receptors are the largest receptor tyrosine kinase family. Several family members are expressed in hematopoietic cells. Previously, the expression of a member of this family, EphA2, was identified on dendritic like cells in tonsils. We therefore specifically examined the expression of EphA2 on in vitro generated dendritic cells.<br />Results: In this study, expression of the EphA2 receptor was identified on in vitro generated Langerhans like dendritic cells compared to in vitro generated dendritic cells. We show that ligand induced engagement of the EphA2 receptor leads to receptor autophosphorylation indicating a functional receptor signaling pathway in these cells. We also observe phosphorylation and dephosphorylation of distinct proteins following ligand activation of EphA receptors. In co-stimulation assays, receptor-ligand interaction reduces the capacity of the Langerhans like dendritic cells to stimulate resting CD4+ T cells.<br />Conclusion: Engagement of EphA receptor tyrosine kinases on Langerhans like dendritic cells induces signaling as shown by tyrosine phosphorylation and dephosphorylation of distinct proteins. Furthermore this engagement renders the cells less capable of stimulating CD4+ T cells.
- Subjects :
- Antigens, Surface biosynthesis
CD4-Positive T-Lymphocytes physiology
Cell Adhesion Molecules biosynthesis
Cell Division physiology
Cell Line
Cross-Linking Reagents pharmacology
Cytokines physiology
Ephrin-A2 physiology
Lymphocyte Activation physiology
Receptor, EphA2 genetics
Receptor, EphA2 metabolism
Receptor, EphA2 physiology
Signal Transduction physiology
T-Lymphocyte Subsets physiology
T-Lymphocytes metabolism
Th1 Cells physiology
Th2 Cells physiology
Dendritic Cells chemistry
Dendritic Cells metabolism
Gene Expression Regulation, Enzymologic genetics
Langerhans Cells chemistry
Langerhans Cells metabolism
Receptors, Eph Family genetics
Receptors, Eph Family physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1471-2172
- Volume :
- 5
- Database :
- MEDLINE
- Journal :
- BMC immunology
- Publication Type :
- Academic Journal
- Accession number :
- 15176971
- Full Text :
- https://doi.org/10.1186/1471-2172-5-9