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Complex chromosomal rearrangements induced in vivo by heavy ions.
- Source :
-
Cytogenetic and genome research [Cytogenet Genome Res] 2004; Vol. 104 (1-4), pp. 240-4. - Publication Year :
- 2004
-
Abstract
- It has been suggested that the ratio complex/simple exchanges can be used as a biomarker of exposure to high-LET radiation. We tested this hypothesis in vivo, by considering data from several studies that measured complex exchanges in peripheral blood from humans exposed to mixed fields of low- and high-LET radiation. In particular, we studied data from astronauts involved in long-term missions in low-Earth-orbit, and uterus cancer patients treated with accelerated carbon ions. Data from two studies of chromosomal aberrations in astronauts used blood samples obtained before and after space flight, and a third study used blood samples from patients before and after radiotherapy course. Similar methods were used in each study, where lymphocytes were stimulated to grow in vitro, and collected after incubation in either colcemid or calyculin A. Slides were painted with whole-chromosome DNA fluorescent probes (FISH), and complex and simple chromosome exchanges in the painted genome were classified separately. Complex-type exchanges were observed at low frequencies in control subjects, and in our test subjects before the treatment. No statistically significant increase in the yield of complex-type exchanges was induced by the space flight. Radiation therapy induced a high fraction of complex exchanges, but no significant differences could be detected between patients treated with accelerated carbon ions or X-rays. Complex chromosomal rearrangements do not represent a practical biomarker of radiation quality in our test subjects.<br /> (Copyright 2003 S. Karger AG, Basel)
- Subjects :
- Aged
Aged, 80 and over
Astronauts
Biomarkers
Breast Neoplasms blood
Breast Neoplasms surgery
Carbon
Carcinoma, Squamous Cell blood
Carcinoma, Squamous Cell radiotherapy
Chromatids radiation effects
Chromatids ultrastructure
Chromosome Painting
Chromosomes, Human ultrastructure
Cosmic Radiation adverse effects
Esophageal Neoplasms blood
Female
Humans
Ions therapeutic use
Linear Energy Transfer
Male
Middle Aged
Occupational Exposure
Uterine Cervical Neoplasms blood
Uterine Cervical Neoplasms radiotherapy
X-Rays adverse effects
Chromosome Aberrations
Chromosome Breakage
Chromosomes, Human radiation effects
Ions adverse effects
Subjects
Details
- Language :
- English
- ISSN :
- 1424-859X
- Volume :
- 104
- Issue :
- 1-4
- Database :
- MEDLINE
- Journal :
- Cytogenetic and genome research
- Publication Type :
- Academic Journal
- Accession number :
- 15162046
- Full Text :
- https://doi.org/10.1159/000077497