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Rewiring cell adhesion.
- Source :
-
Journal of the American Chemical Society [J Am Chem Soc] 2004 Jun 02; Vol. 126 (21), pp. 6504-5. - Publication Year :
- 2004
-
Abstract
- The adhesion of cells is mediated by the binding of several cell-surface receptors to ligands found in the extracellular matrix. These receptors often have overlapping specificities for the peptide ligands, making it difficult to understand the roles for discrete receptors in cell adhesion, migration, and differentiation as well as to direct the selective adhesion of cell types in tissue-engineering applications. To overcome these limitations, we developed a strategy to rewire the receptor-ligand interactions between a cell and substrate to ensure that adhesion is mediated by a single receptor with unique specificity. The strategy combines a genetic approach to engineer the cell surface with a chimeric integrin receptor having a unique ligand binding domain with a surface chemistry approach to prepare substrates that present ligands that are bound by the new binding domain. We show that Chinese hamster ovary cells that are engineered with a chimeric beta1 integrin adhere, signal, and even migrate on a synthetic matrix.
- Subjects :
- Animals
CHO Cells
Carbonic Anhydrase IV antagonists & inhibitors
Carbonic Anhydrase IV chemistry
Carbonic Anhydrase IV genetics
Carbonic Anhydrase Inhibitors chemistry
Carbonic Anhydrase Inhibitors pharmacology
Cell Movement
Chimerin Proteins chemistry
Chimerin Proteins genetics
Cricetinae
Focal Adhesion Protein-Tyrosine Kinases
Integrin alpha5beta1 chemistry
Integrin alpha5beta1 genetics
Oligopeptides chemistry
Oligopeptides metabolism
Phosphorylation
Protein Engineering methods
Protein-Tyrosine Kinases metabolism
Sulfonamides chemistry
Sulfonamides pharmacology
Surface Properties
Transfection
Benzenesulfonamides
Carbonic Anhydrase IV physiology
Cell Adhesion physiology
Integrin alpha5beta1 physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0002-7863
- Volume :
- 126
- Issue :
- 21
- Database :
- MEDLINE
- Journal :
- Journal of the American Chemical Society
- Publication Type :
- Academic Journal
- Accession number :
- 15161249
- Full Text :
- https://doi.org/10.1021/ja039058e