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CD44-chondroitin sulfate interactions mediate leukocyte rolling under physiological flow conditions.
- Source :
-
Immunology letters [Immunol Lett] 2004 May 15; Vol. 93 (2-3), pp. 163-70. - Publication Year :
- 2004
-
Abstract
- CD44 on leukocytes binds to its glycosaminoglycan (GAG) ligand, hyaluronic acid, and mediates the rolling of leukocytes on vascular endothelial cells. We previously reported that the recombinant CD44 protein binds to other GAGs, including chondroitin sulfates (CS), although the physiological significance of this interaction has remained unclear. Here we report that the CD44 expressed on mouse lymphoma BW5147 cells supports cell binding to immobilized CS under static conditions and mediates cell rolling in CS-coated glass capillary tubes under shear stresses ranging from 0.5 to 1.5 dyn/cm(2), which is within the physiological range of forces in venules. Both interactions were completely inhibited by pretreating the cells with an anti-CD44 antibody or by pretreating the CS with chondroitinase ABC, but not hyaluronidase. To address the role of the CD44-CS interaction in vivo, we examined the tissue localization of the CS that interacts with CD44. Interestingly, a recombinant CD44 fusion protein bound to hepatic sinuosoidal endothelial cells where CS was also expressed, as assessed by immunohistochemistry. These findings support the involvement of the CD44-CS interaction in the primary adhesion of lymphocytes to endothelial cells and raise the possibility that this interaction plays a role in the capture of CD44-positive cells, such as activated T cells and certain tumor cells, by the hepatic sinusoidal vasculature.
- Subjects :
- Animals
Antibodies, Monoclonal immunology
Antibodies, Monoclonal pharmacology
Cell Adhesion drug effects
Cell Adhesion physiology
Cell Line, Tumor
Chondroitin metabolism
Chondroitin Sulfates analysis
Chondroitinases and Chondroitin Lyases metabolism
Endothelium chemistry
Heparitin Sulfate metabolism
Hyaluronan Receptors immunology
Hyaluronic Acid metabolism
Hyaluronoglucosaminidase metabolism
Keratan Sulfate metabolism
Leukocyte Rolling drug effects
Liver chemistry
Mice
Mice, Inbred C57BL
Microfluidics methods
Microscopy, Fluorescence
Microscopy, Immunoelectron
Microvilli chemistry
Protein Binding
T-Lymphocytes chemistry
T-Lymphocytes metabolism
T-Lymphocytes physiology
Venules chemistry
Chondroitin Sulfates metabolism
Hyaluronan Receptors metabolism
Leukocyte Rolling physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0165-2478
- Volume :
- 93
- Issue :
- 2-3
- Database :
- MEDLINE
- Journal :
- Immunology letters
- Publication Type :
- Academic Journal
- Accession number :
- 15158613
- Full Text :
- https://doi.org/10.1016/j.imlet.2004.03.013