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Interaction of pathogenic bacteria with rabbit appendix M cells: bacterial motility is a key feature in vivo.
- Source :
-
Microbes and infection [Microbes Infect] 2004 May; Vol. 6 (6), pp. 521-8. - Publication Year :
- 2004
-
Abstract
- Rabbit appendix consists mainly of lymphoid follicles (LF) covered by M cells, the specialized antigen-sampling cells of the mucosal immune system, and surrounded by glandular epithelium. Until now, these M cells have been characterized morphologically and histologically by using cellular markers. Here, the adhesion and transport of pathogenic bacteria were investigated to assess the function of M cells of the appendix. We used the enteroinvasive motile Salmonella typhimurium and the rabbit enteropathogenic non-motile Escherichia coli RDEC-1, which are known to target specifically rabbit M cells of Peyer's patches (PPs). We found that S. typhimurium efficiently attached and was transported through appendix M cells in vivo. In contrast to S. typhimurium, RDEC-1 targeted M cells only ex vivo, when bacteria were allowed to have direct contact with the surface of the follicle. The difference in interaction of the two bacteria with appendix M cells led us to investigate whether this could be correlated with the lack of motility of RDEC-1. We used an aflagellate mutant of S. typhimurium and found that it had the same infection phenotype as RDEC-1. Gene complementation restored the efficiency of infection to that of S. typhimurium wild-type strain. In conclusion, we show that M cells of the appendix display features of the canonical M cells of PP, since they efficiently sample luminal pathogenic bacteria. However, due to the morphology of the appendix, motile bacteria appear to be more potent in their interactions with appendix M cells.
- Subjects :
- Animals
Appendix cytology
Bacterial Adhesion
Escherichia coli immunology
Escherichia coli pathogenicity
Escherichia coli Infections immunology
Escherichia coli Infections microbiology
Flagella genetics
Flagella physiology
Gene Deletion
Genetic Complementation Test
Immunohistochemistry
Lymphoid Tissue cytology
Lymphoid Tissue physiology
Lymphoid Tissue ultrastructure
Microscopy, Electron, Scanning
Movement
Mutation
Peyer's Patches microbiology
Rabbits
Salmonella Infections, Animal immunology
Salmonella Infections, Animal microbiology
Salmonella typhimurium immunology
Salmonella typhimurium pathogenicity
Appendix immunology
Appendix microbiology
Bacteria immunology
Bacteria pathogenicity
Intestinal Mucosa cytology
Lymphoid Tissue microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1286-4579
- Volume :
- 6
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Microbes and infection
- Publication Type :
- Academic Journal
- Accession number :
- 15158184
- Full Text :
- https://doi.org/10.1016/j.micinf.2004.02.009