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Ex-vivo study of flow dynamics and endothelial cell structure during extended hypothermic machine perfusion preservation of livers.
- Source :
-
Cryobiology [Cryobiology] 2004 Jun; Vol. 48 (3), pp. 322-32. - Publication Year :
- 2004
-
Abstract
- Liver transplantation is often the only effective treatment for end stage liver diseases resulting from cirrhosis, hepatitis, progressive jaundice, and biliary atresia. Hypothermic machine perfusion (HMP) preservation may enhance donor pool by extending preservation time and reclaiming marginal donor livers including those from non-heart beating donors (NHBD), as demonstrated in the kidney. However, current HMP protocols have not been successful in improving extended preservation of livers and the major cause of preservation injury remains unknown. An intravital microscopy study was conducted to understand the flow dynamics of sinusoidal perfusion during 24h HMP with cold modified University of Wisconsin (UW) solution. Fluorescein isothiocynate (FITC) labeled albumin was utilized to visualize microvascular space and FITC labeled red blood cells (RBCs) were used to visualize flow dynamics during HMP. A heterogeneous flow pattern with regions of red cell stasis was observed after 24-h HMP. To examine the cause of red cell stasis, intravital and confocal microscopy studies of endothelial cells (ECs) structure labeled with DiI acetylated low-density lipoprotein (DiI acLDL) were conducted. These studies suggest that morphological changes in EC structures occurred during 24h HMP, which may cause obstruction to the sinusoidal flow. Histological findings confirm these results. As a result, heterogeneous flow pattern, red cell stasis, and edema occur, which may lead to the failure of these tissues following extended HMP.
- Subjects :
- Adenosine
Albumins
Allopurinol
Animals
Erythrocytes ultrastructure
Glutathione
Hot Temperature
Insulin
Liver Circulation
Male
Microcirculation ultrastructure
Microscopy, Confocal
Microscopy, Fluorescence
Organ Preservation Solutions
Perfusion instrumentation
Raffinose
Rats
Rats, Sprague-Dawley
Rheology
Spectroscopy, Fourier Transform Infrared
Time Factors
Cryopreservation methods
Endothelial Cells ultrastructure
Liver
Organ Preservation methods
Perfusion methods
Subjects
Details
- Language :
- English
- ISSN :
- 0011-2240
- Volume :
- 48
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Cryobiology
- Publication Type :
- Academic Journal
- Accession number :
- 15157780
- Full Text :
- https://doi.org/10.1016/j.cryobiol.2004.01.010