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Detection rate and antigenic specificities of antineutrophil cytoplasmic antibodies in chinese patients with clinically suspected vasculitis.
- Source :
-
Clinical and diagnostic laboratory immunology [Clin Diagn Lab Immunol] 2004 May; Vol. 11 (3), pp. 559-62. - Publication Year :
- 2004
-
Abstract
- The detection rate of antineutrophil cytoplasmic antibodies (ANCA) in Chinese patients with clinically suspected small vessel vasculitis was investigated, and their antigen specificity and demographic features were analyzed. A number of sera (n = 5,604) sent to our referral laboratory for ANCA screening were tested by indirect immunofluorescence (IIF), enzyme-linked immunosorbent assays (ELISAs) for myeloperoxidase (MPO)- and proteinase 3 (PR3)-ANCA. Then the IIF-ANCA-positive sera that were negative for MPO- and PR3-ANCA were further tested by antigen-specific ELISA by using other five highly purified known ANCA antigens as solid-phase ligands. The known antigens included bactericidal/permeability-increasing protein (BPI), human leukocyte elastase (HLE), lactoferrin, cathepsin G, and azurocidins. Of the 5,604 sera, 267 (4.76%) sera were IIF-ANCA positive and 390 (7%) were antinuclear antibody (ANA) positive in the IIF assay. Of the IIF-positive samples, 213 were anti-MPO positive, 32 were anti-PR3 positive, and five cases were positive for both. Of the 48 sera positive for IIF-ANCA but negative for MPO- and PR3-ANCA, 13 sera (27%) recognized other target antigens, 7 sera recognized BPI, 5 recognized HLE, 1 recognize cathepsin G, and 1 recognized azurocidin. None of the sera recognized lactoferrin, and one serum sample recognized both BPI and HLE. The majority of ANCA-positive patients presented in summer or winter. There was no difference in gender (male/female ratio, 1:1.12) in ANCA-positive patients with a mean age of 53.1 years. The male/female ratio was 1.17:1 for patients over 60 years of age; however, it was 1:4 for patients under 20 years of age. We conclude that ANCA-related diseases are not rare in China, and the major antigens are MPO and PR3. When the IIF technique is used to detect ANCA, ANA should be carefully distinguished.
- Subjects :
- Adolescent
Adult
Aged
Anemia, Hemolytic blood
Anemia, Hemolytic complications
Anemia, Hemolytic immunology
Antibodies, Antineutrophil Cytoplasmic blood
Antibodies, Antinuclear blood
Antibody Specificity immunology
Antimicrobial Cationic Peptides
Autoantibodies blood
Autoantibodies immunology
Blood Proteins immunology
Cathepsin G
Cathepsins immunology
Child
China epidemiology
Colitis, Ulcerative blood
Colitis, Ulcerative complications
Colitis, Ulcerative immunology
Enzyme-Linked Immunosorbent Assay
Female
Fluorescent Antibody Technique, Indirect
Granulomatosis with Polyangiitis blood
Granulomatosis with Polyangiitis complications
Granulomatosis with Polyangiitis immunology
Humans
Lactoferrin immunology
Leukocyte Elastase immunology
Male
Membrane Proteins immunology
Middle Aged
Myeloblastin
Peroxidase immunology
Seasons
Serine Endopeptidases immunology
Sex Factors
Vasculitis epidemiology
Vasculitis etiology
Antibodies, Antineutrophil Cytoplasmic immunology
Vasculitis immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1071-412X
- Volume :
- 11
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Clinical and diagnostic laboratory immunology
- Publication Type :
- Academic Journal
- Accession number :
- 15138182
- Full Text :
- https://doi.org/10.1128/CDLI.11.3.559-562.2004