Back to Search
Start Over
Both ApxI and ApxII of Actinobacillus pleuropneumoniae serotype 1 are necessary for full virulence.
- Source :
-
Veterinary microbiology [Vet Microbiol] 2004 May 20; Vol. 100 (1-2), pp. 17-23. - Publication Year :
- 2004
-
Abstract
- Most serotypes of A. pleuropneumoniae produce more than one toxin in vivo. To determine the value of the production of more than one toxin in the development of disease, we tested the pathogenicity of isogenic strains of A. pleuropneumoniae serotype 1 that are mutated in the toxin genes apxIA and/or apxIIA or in the transport genes apxIBD. Bacteria mutated in both apxIA and apxIIA, or in apxIBD, were unable to induce pathological lesions, thereby confirming the conclusion that ApxI and ApxII are essential for the pathogenesis of pleuropneumonia. Infection with isogenic strains lacking either ApxI or ApxII did not consistently lead to pleuropneumonia unlike the parent strain S4074. ApxII seemed at least as important as ApxI for the development of clinical and pathological symptoms. Only one of the four pigs inoculated with a mutant strain unable to produce ApxII developed mild pneumonia whereas two out of the three pigs inoculated with a mutant strain unable to produce ApxI developed more severe lesions. The results indicate that both ApxI and ApxII of A. pleuropneumoniae serotype 1 are necessary for full virulence.
- Subjects :
- Actinobacillus Infections microbiology
Actinobacillus Infections pathology
Actinobacillus pleuropneumoniae genetics
Animals
Bacterial Proteins chemistry
Bacterial Proteins genetics
Body Temperature
Cytokines blood
Hemolysin Proteins
Histocytochemistry veterinary
Leukocyte Count veterinary
Lung microbiology
Lung pathology
Mutagenesis, Site-Directed
Pleuropneumonia microbiology
Pleuropneumonia pathology
Random Allocation
Swine
Virulence
Actinobacillus Infections veterinary
Actinobacillus pleuropneumoniae pathogenicity
Bacterial Proteins physiology
Pleuropneumonia veterinary
Swine Diseases microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 0378-1135
- Volume :
- 100
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Veterinary microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 15135509
- Full Text :
- https://doi.org/10.1016/j.vetmic.2003.09.024