Back to Search Start Over

[Inducing peripheral materno-fetal immuno-tolerance by blockade of costimulatory signal at early stage of gestation of the abortion-prone murine model].

Authors :
Jin LP
Li DJ
Wang MY
Zhu XY
Zhu Y
Meng Y
Yuan MM
Source :
Zhonghua fu chan ke za zhi [Zhonghua Fu Chan Ke Za Zhi] 2004 Apr; Vol. 39 (4), pp. 234-7.
Publication Year :
2004

Abstract

Objective: To study effect of blockade of costimulatory signal CD(80), CD(86) at the early stage of gestation on the maternal peripheral immuno-tolerance status to paternal antigen and pregnant outcome of murine abortion-prone model.<br />Methods: The experiments were performed in two groups, using CBA/J x BALB/c matings as the normal pregnancy model and CBA/J x DBA/2 matings as the abortion-prone model. The pregnant CBA/J mice which had mated with DBA/2 or BALB/c male mice respectively were injected intraperitoneally with purified rat isotype IgG or purified rat anti-mouse CD(80), CD(86) mAb at day 4 of gestation (time of implantation). The proliferation response of maternal splenic immuno-competent cells to paternal splenic cells as stimulator was analyzed by one way mixed lymphocyte reaction, and IL-2 production was assayed by ELISA to evaluate the immuno-tolerance of maternal peripheral immuno-competent cells to paternal antigen at day 9 of gestation. The embryo resorption rate was counted at day 14 of gestation.<br />Results: In the CBA/J x DBA/2 matings, anti-CD(80), CD(86) mAb administered at day 4 of gestation reduced significantly the embryo resorption rate, and induced a significantly lower proliferation response and IL-2 production by maternal splenic immuno-competent cells to paternal antigen compared with rat isotype IgG.<br />Conclusions: In vivo blockade of costimulatory signal at day 4 of gestation can induce the immuno-tolerance of maternal peripheral immuno-competent cells to paternal antigen, thus leading the embryo resorption rate of the abortion-prone model to that of normal pregnancy model.

Details

Language :
Chinese
ISSN :
0529-567X
Volume :
39
Issue :
4
Database :
MEDLINE
Journal :
Zhonghua fu chan ke za zhi
Publication Type :
Academic Journal
Accession number :
15130348