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The Filamin-A is a partner of Tc-mip, a new adapter protein involved in c-maf-dependent Th2 signaling pathway.
- Source :
-
Molecular immunology [Mol Immunol] 2004 Mar; Vol. 40 (17), pp. 1257-61. - Publication Year :
- 2004
-
Abstract
- Using a yeast two-hybrid screen, we identified Filamin-A as a binding partner of the new adapter protein c-mip (c-maf inducing protein) and it's splice variant Tc-mip (truncated c-maf inducing protein). We have previously shown that Tc-mip is involved in Th2 signaling pathway and cytoskeletal reorganization in patients with minimal change nephrotic syndrome (MCNS), the most frequent glomerular disease in children. We showed that Filamin-A and c-mip or Tc-mip co-immunoprecipitate from c-mip or Tc-mip Jurkat transfected cells using antibodies directed against both types of proteins. In co-immunoprecipitate Jurkat cells, Filamin-A and c-mip were distributed evenly in the cytoplasm, whereas in Tc-mip-transfected Jurkat cells, Filamin-A was expressed in zones facing the cell contact. Moreover, we found that Filamin-A was upregulated in T lymphocytes of MCNS patients, as compared to normal subjects. These findings suggest that Filamin-A interacts with c-mip/Tc-mip in this new T-cell signaling pathway.
- Subjects :
- Adaptor Proteins, Signal Transducing
Filamins
Humans
Jurkat Cells
Nephrosis, Lipoid metabolism
Precipitin Tests
Proto-Oncogene Proteins c-maf
Contractile Proteins metabolism
Cytoskeletal Proteins metabolism
DNA-Binding Proteins metabolism
Microfilament Proteins metabolism
Proto-Oncogene Proteins metabolism
Signal Transduction immunology
Th2 Cells metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0161-5890
- Volume :
- 40
- Issue :
- 17
- Database :
- MEDLINE
- Journal :
- Molecular immunology
- Publication Type :
- Academic Journal
- Accession number :
- 15128042
- Full Text :
- https://doi.org/10.1016/j.molimm.2003.11.035