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The Filamin-A is a partner of Tc-mip, a new adapter protein involved in c-maf-dependent Th2 signaling pathway.

Authors :
Grimbert P
Valanciute A
Audard V
Lang P
Guellaƫn G
Sahali D
Source :
Molecular immunology [Mol Immunol] 2004 Mar; Vol. 40 (17), pp. 1257-61.
Publication Year :
2004

Abstract

Using a yeast two-hybrid screen, we identified Filamin-A as a binding partner of the new adapter protein c-mip (c-maf inducing protein) and it's splice variant Tc-mip (truncated c-maf inducing protein). We have previously shown that Tc-mip is involved in Th2 signaling pathway and cytoskeletal reorganization in patients with minimal change nephrotic syndrome (MCNS), the most frequent glomerular disease in children. We showed that Filamin-A and c-mip or Tc-mip co-immunoprecipitate from c-mip or Tc-mip Jurkat transfected cells using antibodies directed against both types of proteins. In co-immunoprecipitate Jurkat cells, Filamin-A and c-mip were distributed evenly in the cytoplasm, whereas in Tc-mip-transfected Jurkat cells, Filamin-A was expressed in zones facing the cell contact. Moreover, we found that Filamin-A was upregulated in T lymphocytes of MCNS patients, as compared to normal subjects. These findings suggest that Filamin-A interacts with c-mip/Tc-mip in this new T-cell signaling pathway.

Details

Language :
English
ISSN :
0161-5890
Volume :
40
Issue :
17
Database :
MEDLINE
Journal :
Molecular immunology
Publication Type :
Academic Journal
Accession number :
15128042
Full Text :
https://doi.org/10.1016/j.molimm.2003.11.035