Back to Search
Start Over
Positive control of swarming, rhamnolipid synthesis, and lipase production by the posttranscriptional RsmA/RsmZ system in Pseudomonas aeruginosa PAO1.
- Source :
-
Journal of bacteriology [J Bacteriol] 2004 May; Vol. 186 (10), pp. 2936-45. - Publication Year :
- 2004
-
Abstract
- In Pseudomonas aeruginosa, the small RNA-binding, regulatory protein RsmA is a negative control element in the formation of several extracellular products (e.g., pyocyanin, hydrogen cyanide, PA-IL lectin) as well as in the production of N-acylhomoserine lactone quorum-sensing signal molecules. RsmA was found to control positively the ability to swarm and to produce extracellular rhamnolipids and lipase, i.e., functions contributing to niche colonization by P. aeruginosa. An rsmA null mutant was entirely devoid of swarming but produced detectable amounts of rhamnolipids, suggesting that factors in addition to rhamnolipids influence the swarming ability of P. aeruginosa. A small regulatory RNA, rsmZ, which antagonized the effects of RsmA, was identified in P. aeruginosa. Expression of the rsmZ gene was dependent on both the global regulator GacA and RsmA, increased with cell density, and was subject to negative autoregulation. Overexpression of rsmZ and a null mutation in rsmA resulted in quantitatively similar, negative or positive effects on target genes, in agreement with a model that postulates titration of RsmA protein by RsmZ RNA.
- Subjects :
- Amino Acid Sequence
Bacterial Proteins antagonists & inhibitors
Base Sequence
Cloning, Molecular
Molecular Sequence Data
Pseudomonas aeruginosa genetics
RNA-Binding Proteins antagonists & inhibitors
Repressor Proteins antagonists & inhibitors
Bacterial Proteins physiology
Gene Expression Regulation, Bacterial
Glycolipids biosynthesis
Lipase biosynthesis
Pseudomonas aeruginosa metabolism
RNA, Bacterial physiology
RNA-Binding Proteins physiology
Repressor Proteins physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9193
- Volume :
- 186
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Journal of bacteriology
- Publication Type :
- Academic Journal
- Accession number :
- 15126453
- Full Text :
- https://doi.org/10.1128/JB.186.10.2936-2945.2004