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Differential expression and dendritic transcript localization of Shank family members: identification of a dendritic targeting element in the 3' untranslated region of Shank1 mRNA.
- Source :
-
Molecular and cellular neurosciences [Mol Cell Neurosci] 2004 May; Vol. 26 (1), pp. 182-90. - Publication Year :
- 2004
-
Abstract
- Shank proteins are scaffolding proteins in the postsynaptic density of excitatory synapses in the mammalian brain. In situ hybridization revealed that Shank1/SSTRIP and Shank2/ProSAP1 mRNAs are widely expressed early in postnatal brain development whereas Shank3/ProSAP2 expression increases during postnatal development especially in the cerebellum and thalamus. Shank1 and Shank3 (but not Shank2) mRNAs are present in the molecular layers of the hippocampus, consistent with a dendritic transcript localization. Shank1 and Shank2 transcripts are detectable in the dendritic fields of Purkinje cells, whereas Shank3 mRNA is restricted to cerebellar granule cells. The appearance of dendritic Shank mRNAs in cerebellar Purkinje cells coincides with the onset of dendrite formation. Expression of reporter transcripts in hippocampal neurons identifies a 200-nucleotide dendritic targeting element (DTE) in the Shank1 mRNA. The widespread presence of Shank mRNAs in dendrites suggests a role for local synthesis of Shanks in response to stimuli that induce alterations in synaptic morphology.
- Subjects :
- Animals
Brain cytology
Brain growth & development
Cerebellar Cortex cytology
Cerebellar Cortex growth & development
Cerebellar Cortex metabolism
Gene Expression Regulation, Developmental genetics
Genes, Regulator genetics
Genes, Reporter genetics
Hippocampus cytology
Hippocampus growth & development
Hippocampus metabolism
Nerve Tissue Proteins genetics
Protein Transport genetics
RNA, Messenger metabolism
Rats
Synaptic Membranes genetics
Synaptic Transmission genetics
3' Untranslated Regions genetics
Adaptor Proteins, Signal Transducing
Brain metabolism
Carrier Proteins genetics
Dendrites metabolism
RNA, Messenger genetics
Synaptic Membranes metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1044-7431
- Volume :
- 26
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Molecular and cellular neurosciences
- Publication Type :
- Academic Journal
- Accession number :
- 15121189
- Full Text :
- https://doi.org/10.1016/j.mcn.2004.01.009