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Detection of bovine respiratory syncytial virus in experimentally infected balb/c mice.
- Source :
-
Veterinary research [Vet Res] 2004 Mar-Apr; Vol. 35 (2), pp. 189-97. - Publication Year :
- 2004
-
Abstract
- The present study used an RT-nested-PCR and an immunohistochemistry assay to detect bovine respiratory syncytial virus in tissues from experimentally infected balb/c mice. As a first step, Chicken Embryo Related (CER) cell monolayers infected with the BRSV-25-BR strain isolated in Brazil were used for antigen production. Then, the infected lung and tracheal tissues of female balb/c mice were collected on 3, 5, 7 and 10 days post-infection and submitted to both techniques. Primers specific to F and G genes that amplify fragments of 481 bp and 371 bp, respectively, were used. The BRSV detection was not successful in all of the animals tested. The genomic fragment of the G gene from the organs of some infected mice on all analyzed post-infection days was amplified. However, in the RT-nested-PCR corresponding to the F gene, it was not possible to observe any amplified fragment. This was probably due to the higher sensitivity of the developed technique to amplify the fragment corresponding to the G gene compared to the F gene. Moreover, only three of the lungs collected five days post-infection were positive by immunohistochemistry. To the author's knowledge, this is the first study reporting bovine respiratory syncytial virus detection in balb/c mice after experimental inoculation.
- Subjects :
- Animals
Cattle
Chick Embryo virology
DNA, Viral analysis
Female
Immunohistochemistry veterinary
Mice
Mice, Inbred BALB C
Respiratory Syncytial Virus Infections virology
Respiratory Syncytial Virus, Bovine genetics
Reverse Transcriptase Polymerase Chain Reaction veterinary
Sensitivity and Specificity
Specific Pathogen-Free Organisms
Cattle Diseases virology
Respiratory Syncytial Virus Infections veterinary
Respiratory Syncytial Virus, Bovine isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 0928-4249
- Volume :
- 35
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Veterinary research
- Publication Type :
- Academic Journal
- Accession number :
- 15099495
- Full Text :
- https://doi.org/10.1051/vetres:2004003