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A new method for determining the local environment and orientation of individual side chains of membrane-binding peptides.
- Source :
-
Journal of the American Chemical Society [J Am Chem Soc] 2004 Apr 28; Vol. 126 (16), pp. 5078-9. - Publication Year :
- 2004
-
Abstract
- We studied here the binding of the mastoparan X peptide to a zwitterionic lipid bilayer (POPC) and demonstrated that nitrile-derivatized amino acids can be used to determine the hydration state (or change in hydration state) of specific sites of membrane-interactive peptides (upon binding). We have also shown that polarized ATR-FTIR measurements can further be used to uncover information regarding the spatial orientation of individual side chains as well as their conformational preference within the lipid bilayer.
Details
- Language :
- English
- ISSN :
- 0002-7863
- Volume :
- 126
- Issue :
- 16
- Database :
- MEDLINE
- Journal :
- Journal of the American Chemical Society
- Publication Type :
- Academic Journal
- Accession number :
- 15099085
- Full Text :
- https://doi.org/10.1021/ja032015d