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Detection and quantitation of hepatitis E virus in human faeces by real-time quantitative PCR.
- Source :
-
Journal of virological methods [J Virol Methods] 2004 Jun 15; Vol. 118 (2), pp. 77-82. - Publication Year :
- 2004
-
Abstract
- Hepatitis E Virus (HEV) is the causative agent of an acute and self-limited form of hepatitis. The virus is transmitted by the faecal-oral route and is a major cause of viral hepatitis in much of the developing world where it causes sporadic infections and large-scale epidemics. A simple and rapid protocol for the measurement of HEV faecal shedding by a real-time polymerase chain reaction (PCR) with the SYBR Green method on a LightCycler instrument, is described. After only 3h the real-time quantitative PCR method detected 10 molecules of HEV cDNA fragment per reaction tube and showed a high linear dynamic range of quantitation (10-10(6) molecules of cDNA/reaction) with a good correlation (r = -1.00). Its specificity was confirmed by assay in human faecal samples.
- Subjects :
- Benzothiazoles
DNA, Complementary genetics
Diamines
Humans
Organic Chemicals metabolism
Polymerase Chain Reaction instrumentation
Quinolines
Sensitivity and Specificity
Feces virology
Hepatitis E virology
Hepatitis E virus genetics
Hepatitis E virus isolation & purification
Polymerase Chain Reaction methods
Reverse Transcriptase Polymerase Chain Reaction methods
Subjects
Details
- Language :
- English
- ISSN :
- 0166-0934
- Volume :
- 118
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of virological methods
- Publication Type :
- Academic Journal
- Accession number :
- 15081602
- Full Text :
- https://doi.org/10.1016/j.jviromet.2004.01.025