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Identification of putative voltage-dependent Ca2+-permeable channels involved in cryptogein-induced Ca2+ transients and defense responses in tobacco BY-2 cells.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2004 May 07; Vol. 317 (3), pp. 823-30. - Publication Year :
- 2004
-
Abstract
- Ca(2+) is the pivotal second messenger for induction of defense responses induced by treatment of pathogen-derived elicitor or microbial infection in plants. However, molecular bases for elicitor-induced generation of Ca(2+) signals (Ca(2+) transients) are largely unknown. We here identified cDNAs for putative voltage-dependent Ca(2+)-permeable channels, NtTPC1A and NtTPC1B, that are homologous to TPC1 (two pore channel) from suspension-cultured tobacco BY-2 cells. NtTPC1s complemented the growth of a Saccharomyces cerevisiae mutant defective in CCH1, a putative Ca(2+) channel, in a low Ca(2+) medium, suggesting that both products permeate Ca(2+) through the plasma membrane. Cosuppression of NtTPC1s in apoaequorin-expressing BY-2 cells resulted in inhibition of rise in cytosolic free Ca(2+) concentration ([Ca(2+)](cyt)) in response to sucrose and a fungal elicitor cryptogein, while it did not affect hypoosmotic shock-induced [Ca(2+)](cyt) increase. Cosuppression of NtTPC1s also caused suppression of cryptogein-induced programmed cell death and defense-related gene expression. These results suggest that NtTPC1s are involved in Ca(2+) mobilization induced by the cryptogein and sucrose, and have crucial roles in cryptogein-induced signal transduction pathway.
- Subjects :
- Amino Acid Sequence
Base Sequence
Calcium Channels chemistry
Calcium Channels drug effects
Calcium Channels genetics
Cell Line
Cloning, Molecular
DNA Primers
Fungal Proteins
Molecular Sequence Data
Saccharomyces cerevisiae growth & development
Sequence Homology, Amino Acid
Nicotiana cytology
Algal Proteins pharmacology
Calcium physiology
Calcium Channels physiology
Nicotiana physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 317
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 15081414
- Full Text :
- https://doi.org/10.1016/j.bbrc.2004.03.114