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Golgi targeting of ARF-like GTPase Arl3p requires its Nalpha-acetylation and the integral membrane protein Sys1p.
- Source :
-
Nature cell biology [Nat Cell Biol] 2004 May; Vol. 6 (5), pp. 414-9. Date of Electronic Publication: 2004 Apr 11. - Publication Year :
- 2004
-
Abstract
- Myristoylation of ARF family GTPases is required for their association with Golgi and endosomal membranes, where they regulate protein sorting and the lipid composition of these organelles. The Golgi-localized ARF-like GTPase Arl3p/ARP lacks a myristoylation signal, indicating that its targeting mechanism is distinct from myristoylated ARFs. We demonstrate that acetylation of the N-terminal methionine of Arl3p requires the NatC N(alpha)-acetyltransferase and that this modification is required for its Golgi localization. Chemical crosslinking and fluorescence microscopy experiments demonstrate that localization of Arl3p also requires Sys1p, a Golgi-localized integral membrane protein, which may serve as a receptor for acetylated Arl3p.
- Subjects :
- ADP-Ribosylation Factors genetics
Acetylation
Arylamine N-Acetyltransferase genetics
Arylamine N-Acetyltransferase metabolism
N-Terminal Acetyltransferase B
Protein Transport physiology
Recombinant Fusion Proteins genetics
Recombinant Fusion Proteins metabolism
Saccharomyces cerevisiae genetics
Saccharomyces cerevisiae metabolism
Saccharomyces cerevisiae Proteins genetics
Vesicular Transport Proteins
ADP-Ribosylation Factors metabolism
Acetyltransferases metabolism
Golgi Apparatus metabolism
Membrane Proteins metabolism
Saccharomyces cerevisiae Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1465-7392
- Volume :
- 6
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Nature cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 15077114
- Full Text :
- https://doi.org/10.1038/ncb1121