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Synergistic effects between FSH and 1,1-dichloro-2,2-bis(P-chlorophenyl)ethylene (P,P'-DDE) on human granulosa cell aromatase activity.
- Source :
-
Human reproduction (Oxford, England) [Hum Reprod] 2004 May; Vol. 19 (5), pp. 1089-93. Date of Electronic Publication: 2004 Apr 07. - Publication Year :
- 2004
-
Abstract
- Background: 1,1-Dichloro-2,2-bis(P-chlorophenyl)ethylene (P,P'-DDE, DDE), a metabolite of DDT, is a persistent hormonally active environmental toxicant which has been found in human serum and follicular fluid. The objective of this study was to investigate the interaction between FSH and ppDDE on aromatase activity in primary cultures of human granulosa cells.<br />Methods: Granulosa cells were obtained at the time of oocyte retrieval for IVF procedures and cultured in defined medium containing FSH and environmentally relevant concentrations of DDE. Aromatase activity was measured by incubating the cells with 1beta-[(3)H]androstenedione and measuring the release of (3)H(2)O.<br />Results: The granulosa cell response to FSH was highly dependent on the basal level of aromatase activity (r = -0.703, P = 0.001, n = 17) with the highest activity occurring at low basal levels of aromatase activity. Enzyme activity was significantly stimulated at 100 ng DDE/ml. A synergistic effect on aromatizing activity was observed when cells were co-cultured with DDE and FSH.<br />Conclusions: Concentrations of DDE similar to those present in human follicular fluid enhance basal and FSH-stimulated granulosa cell aromatizing enzyme activity.
- Subjects :
- Cells, Cultured
Drug Synergism
Enzyme Activation drug effects
Female
Granulosa Cells cytology
Granulosa Cells enzymology
Humans
Aromatase metabolism
Dichlorodiphenyl Dichloroethylene pharmacology
Follicle Stimulating Hormone pharmacology
Granulosa Cells drug effects
Insecticides pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0268-1161
- Volume :
- 19
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Human reproduction (Oxford, England)
- Publication Type :
- Academic Journal
- Accession number :
- 15070871
- Full Text :
- https://doi.org/10.1093/humrep/deh252