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Diminished expression of sodium transporters in the ureteral obstructed kidney in rats.
- Source :
-
Nephron. Experimental nephrology [Nephron Exp Nephrol] 2004; Vol. 96 (3), pp. e67-76. - Publication Year :
- 2004
-
Abstract
- Background/aims: Whether the postobstructive natriuresis and diuresis is related with an altered regulation of sodium transporters in the kidney was examined.<br />Methods: Male Sprague-Dawley rats underwent either bilateral (BUO) or unilateral obstruction (UUO) of the proximal ureters for 24 h. The expression of Na,K-ATPase, type-3 sodium-hydrogen exchanger (NHE3), type-1 bumetanide-sensitive sodium cotransporter (BSC1), and thiazide-sensitive sodium cotransporter (TSC) proteins was determined in the obstructed kidney by Western blot analysis and immunohistochemistry. Catalytic activity of Na,K-ATPase was also determined.<br />Results: The expression of alpha1 and beta1 subunit proteins and the catalytic activity of Na,K-ATPase were significantly decreased in the obstructed kidney in BUO. The expressions of NHE3, BSC1 and TSC proteins were also significantly decreased. Immunohistochemistry confirmed the downregulation of these sodium transporters in the obstructed kidney. In UUO, the expression of sodium transporters was similarly decreased in the obstructed kidney.<br />Conclusion: The postobstructive natriuresis and diuresis may in part be accounted for by a reduced abundance of sodium transporters in the kidney.<br /> (Copyright 2004 S. Karger AG, Basel)
- Subjects :
- Animals
Carrier Proteins immunology
Carrier Proteins metabolism
Diuresis
Immunohistochemistry
Ligation
Male
Rats
Rats, Sprague-Dawley
Receptors, Drug immunology
Receptors, Drug metabolism
Sodium Chloride Symporters
Sodium-Hydrogen Exchanger 3
Sodium-Hydrogen Exchangers immunology
Sodium-Hydrogen Exchangers metabolism
Sodium-Potassium-Chloride Symporters immunology
Sodium-Potassium-Chloride Symporters metabolism
Sodium-Potassium-Exchanging ATPase immunology
Sodium-Potassium-Exchanging ATPase metabolism
Solute Carrier Family 12, Member 1
Solute Carrier Family 12, Member 3
Ureter surgery
Ion Pumps metabolism
Kidney metabolism
Natriuresis
Symporters
Subjects
Details
- Language :
- English
- ISSN :
- 1660-2129
- Volume :
- 96
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Nephron. Experimental nephrology
- Publication Type :
- Academic Journal
- Accession number :
- 15056983
- Full Text :
- https://doi.org/10.1159/000076748