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Fetal bovine serum influences apoptosis and apoptosis-related gene expression in porcine parthenotes developing in vitro.
- Source :
-
Reproduction (Cambridge, England) [Reproduction] 2004 Jan; Vol. 127 (1), pp. 125-30. - Publication Year :
- 2004
-
Abstract
- This study was conducted to determine the effects of polyvinyl alcohol (PVA), fetal bovine serum (FBS) and bovine serum albumin (BSA) on blastocoel formation, total cell number, apoptosis and Bcl-xL and Bak gene expression in porcine presumptive diploid parthenotes developing in vitro. The addition of 0.4% BSA to the culture medium enhanced the development of 2-cell or late 4-cell stage parthenotes to the blastocyst stage (P < 0.01) while FBS decreased the incidence of blastocoel formation. FBS also reduced the frequency of blastocysts developed from both 2-cell (P < 0.001) and late 4-cell (P < 0.05) embryos and increased the percentage of blastocysts undergoing apoptosis (P < 0.001). The relative abundance of Bcl-xL mRNA in presumptive diploid parthenotes in the control, PVA- and BSA-supplemented medium was similar to that of in vivo-derived embryos, but was significantly higher than in parthenotes cultured with FBS supplement (P < 0.05). Bak mRNA significantly increased at the blastocyst stage in FBS-supplemented cells (P < 0.01). These results suggest that apoptosis-related gene expression is significantly affected by FBS, and that this may result in alteration of apoptosis and embryo viability of porcine embryos developing in vitro.
- Subjects :
- Animals
Cattle
Cell Division
Cells, Cultured
Fertilization in Vitro
Membrane Proteins genetics
Polyvinyl Alcohol pharmacology
Proto-Oncogene Proteins c-bcl-2 genetics
RNA, Messenger analysis
Swine
bcl-2 Homologous Antagonist-Killer Protein
bcl-X Protein
Apoptosis
Blastocyst physiology
Fetal Blood
Gene Expression Regulation
Subjects
Details
- Language :
- English
- ISSN :
- 1470-1626
- Volume :
- 127
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Reproduction (Cambridge, England)
- Publication Type :
- Academic Journal
- Accession number :
- 15056777
- Full Text :
- https://doi.org/10.1530/rep.1.00039