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Effects of sugar inhibition on cellulases and beta-glucosidase during enzymatic hydrolysis of softwood substrates.
- Source :
-
Applied biochemistry and biotechnology [Appl Biochem Biotechnol] 2004 Spring; Vol. 113-116, pp. 1115-26. - Publication Year :
- 2004
-
Abstract
- A quantitative approach was taken to determine the inhibition effects of glucose and other sugar monomers during cellulase and beta-Glucosidase hydrolysis of two types of cellulosic material: Avicel and acetic acid-pretreated softwood. The increased glucose content in the hydrolysate resulted in a dramatic increase in the degrees of inhibition on both beta-Glucosidase and cellulase activities. Supplementation of mannose, xylose, and galactose during cellobiose hydrolysis did not show any inhibitory effects on beta-Glucosidase activity. However, these sugars were shown to have significant inhibitory effects on cellulase activity during cellulose hydrolysis. Our study suggests that high-substrate consistency hydrolysis with supplementation of hemicellulose is likely to be a practical solution to minimizing end-product inhibition effects while producing hydrolysate with high glucose concentration.
Details
- Language :
- English
- ISSN :
- 0273-2289
- Volume :
- 113-116
- Database :
- MEDLINE
- Journal :
- Applied biochemistry and biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 15054257
- Full Text :
- https://doi.org/10.1385/abab:115:1-3:1115