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[DNA analysis in gestational trophoblastic disease].
[DNA analysis in gestational trophoblastic disease].
- Source :
-
Ceska gynekologie [Ceska Gynekol] 2003 Nov; Vol. 68 (6), pp. 442-8. - Publication Year :
- 2003
-
Abstract
- Objective: DNA analysis of different forms of gestational trophoblastic disease.<br />Design: Retrospective clinical study.<br />Setting: Slovak Center of Trophoblastic Disease, Bratislava, Slovak Republic.<br />Methods: In the period of September 1993 to April 2003, eighty-nine cases of gestational trophoblastic disease were analysed. There were 22 cases of partial hydatidiform moles, 58 cases of complete hydatidiform mole, 5 cases of invasive mole and 4 cases of gestational choriocarcinomas. Southern hybridization and polymerase chain reaction were used for DNA analysis.<br />Results: From 22 analyzed cases of partial hydatidiform moles 19 (86.4%) were triploid and 3 (13.6%) diploid ones. There were 58 cases of complete hydatidiform mole and out of them 29 (50%) were homozygous, 28 (48.3%) heterozygous, and in one case (1.7%) both paternal and maternal genome was detected. In 8 cases of heterozygous and in one case of homozygous complete hydatidiform mole occurred a malignant transformation to gestational choriocarcinoma.<br />Conclusions: Molecular analysis can determine the nuclear DNA origin of complete hydatidiform mole and allow us to define the patients with higher risk of malignant transformation usually to gestational choriocarcinoma.
Details
- Language :
- Slovak
- ISSN :
- 1210-7832
- Volume :
- 68
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Ceska gynekologie
- Publication Type :
- Academic Journal
- Accession number :
- 15042856