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Flea-borne transmission model to evaluate vaccine efficacy against naturally acquired bubonic plague.
- Source :
-
Infection and immunity [Infect Immun] 2004 Apr; Vol. 72 (4), pp. 2052-6. - Publication Year :
- 2004
-
Abstract
- A flea-to-mouse transmission model was developed for use in testing new candidate vaccines for the ability to protect against flea-borne plague. The model was used to evaluate a recombinant fusion protein vaccine consisting of the Yersinia pestis F1 and V antigens. After one to three challenges with Y. pestis-infected fleas, 14 of 15 unvaccinated control mice developed plague, with an average septicemia level of 9.2 x 10(8) Y. pestis CFU/ml. None of 15 vaccinated mice developed the disease after similar challenges, and serological testing indicated that transmitted bacteria were eliminated by the immune system before extensive replication and systemic infection could occur. The transmission and development of disease in control mice correlated with the number of bites by blocked fleas but not with the total number of fleabites. The model provides a means to directly assess the efficacy of new vaccines to prevent naturally acquired bubonic plague and to study events at the vector-host interface that lead to dissemination and disease.
- Subjects :
- Animals
Antibodies, Bacterial blood
Antigens, Bacterial immunology
Bacterial Proteins immunology
Disease Models, Animal
Humans
Mice
Mice, Hairless
Plague microbiology
Plague Vaccine immunology
Pore Forming Cytotoxic Proteins
Insect Vectors microbiology
Plague prevention & control
Plague transmission
Plague Vaccine administration & dosage
Siphonaptera microbiology
Yersinia pestis immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0019-9567
- Volume :
- 72
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Infection and immunity
- Publication Type :
- Academic Journal
- Accession number :
- 15039326
- Full Text :
- https://doi.org/10.1128/IAI.72.4.2052-2056.2004