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Human immunoglobulin G mediates protective immunity and identifies protective antigens against larval Strongyloides stercoralis in mice.
- Source :
-
The Journal of infectious diseases [J Infect Dis] 2004 Apr 01; Vol. 189 (7), pp. 1282-90. Date of Electronic Publication: 2004 Mar 12. - Publication Year :
- 2004
-
Abstract
- Protective immunity to larval Strongyloides stercoralis in mice has been shown to be dependent on antibody, complement, and granulocytes. The goals of the present study was to determine the following: (1) whether human serum could passively transfer immunity to mice, (2) the mechanism by which the serum mediated killing, and (3) whether the antigens (Ags) recognized by the protective human antibody could induce protective immunity in mice. Immunoglobulin G (IgG) from a S. stercoralis-seropositive individual passively transferred immunity to mice. The antibody required granulocytes, but not eosinophils, and complement activation to kill the larvae. Antibody-dependent cellular cytotoxicity was not required for larval killing. Immunization of mice with soluble larval Ags isolated by use of the protective immune IgG resulted in protective immunity. In conclusion, immunity could be transferred to mice by IgG from immune humans, and Ags identified by the immune human IgG induced protective immunity in mice, which thereby suggests their possible use in a vaccine against this infection.
- Subjects :
- Animals
Antigens, Helminth isolation & purification
Blotting, Western
Chromatography, Affinity
Complement C3 immunology
Enzyme-Linked Immunosorbent Assay
Humans
Male
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Knockout
Microscopy, Immunoelectron
Receptors, IgG immunology
Strongyloides stercoralis ultrastructure
Strongyloidiasis parasitology
Antigens, Helminth immunology
Immunization, Passive methods
Immunoglobulin G immunology
Strongyloides stercoralis immunology
Strongyloidiasis immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1899
- Volume :
- 189
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- The Journal of infectious diseases
- Publication Type :
- Academic Journal
- Accession number :
- 15031798
- Full Text :
- https://doi.org/10.1086/382484