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Nodal and BMP2/4 signaling organizes the oral-aboral axis of the sea urchin embryo.

Authors :
Duboc V
Röttinger E
Besnardeau L
Lepage T
Source :
Developmental cell [Dev Cell] 2004 Mar; Vol. 6 (3), pp. 397-410.
Publication Year :
2004

Abstract

In the sea urchin embryo, the oral-aboral axis is specified after fertilization by mechanisms that are largely unknown. We report that early sea urchin embryos express Nodal and Antivin in the presumptive oral ectoderm and demonstrate that these genes control formation of the oral-aboral axis. Overexpression of nodal converted the whole ectoderm into oral ectoderm and induced ectopic expression of the orally expressed genes goosecoid, brachyury, BMP2/4, and antivin. Conversely, when the function of Nodal was blocked, by injection of an antisense Morpholino oligonucleotide or by injection of antivin mRNA, neither the oral nor the aboral ectoderm were specified. Injection of nodal mRNA into Nodal-deficient embryos induced an oral-aboral axis in a largely non-cell-autonomous manner. These observations suggest that the mechanisms responsible for patterning the oral-aboral axis of the sea urchin embryo may share similarities with mechanisms that pattern the dorsoventral axis of other deuterostomes.

Details

Language :
English
ISSN :
1534-5807
Volume :
6
Issue :
3
Database :
MEDLINE
Journal :
Developmental cell
Publication Type :
Academic Journal
Accession number :
15030762
Full Text :
https://doi.org/10.1016/s1534-5807(04)00056-5