Back to Search
Start Over
Capturing and profiling adult hair follicle stem cells.
- Source :
-
Nature biotechnology [Nat Biotechnol] 2004 Apr; Vol. 22 (4), pp. 411-7. Date of Electronic Publication: 2004 Mar 14. - Publication Year :
- 2004
-
Abstract
- The hair follicle bulge possesses putative epithelial stem cells. Characterization of these cells has been hampered by the inability to target bulge cells genetically. Here, we use a Keratin1-15 (Krt1-15, also known as K15) promoter to target mouse bulge cells with an inducible Cre recombinase construct or with the gene encoding enhanced green fluorescent protein (EGFP), which allow for lineage analysis and for isolation of the cells. We show that bulge cells in adult mice generate all epithelial cell types within the intact follicle and hair during normal hair follicle cycling. After isolation, adult Krt1-15-EGFP-positive cells reconstituted all components of the cutaneous epithelium and had a higher proliferative potential than Krt1-15-EGFP-negative cells. Genetic profiling of hair follicle stem cells revealed several known and unknown receptors and signaling pathways important for maintaining the stem cell phenotype. Ultimately, these findings provide potential targets for the treatment of hair loss and other disorders of skin and hair.
- Subjects :
- 3T3 Cells
Animals
Cell Division
Cell Line
Cell Lineage
Cell Separation
DNA, Complementary metabolism
Fibroblasts metabolism
Flow Cytometry
Green Fluorescent Proteins
Hair Follicle metabolism
Keratinocytes metabolism
Luminescent Proteins metabolism
Mice
Mice, Transgenic
Oligonucleotide Array Sequence Analysis
Promoter Regions, Genetic
RNA metabolism
RNA, Messenger metabolism
Recombinases metabolism
Reverse Transcriptase Polymerase Chain Reaction
Transcription, Genetic
Up-Regulation
Epithelial Cells cytology
Genetic Techniques
Hair Follicle physiology
Stem Cells cytology
Subjects
Details
- Language :
- English
- ISSN :
- 1087-0156
- Volume :
- 22
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Nature biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 15024388
- Full Text :
- https://doi.org/10.1038/nbt950