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Stochastic rearrangement of immunoglobulin variable-region genes in chicken B-cell development.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 1992 Aug 15; Vol. 89 (16), pp. 7615-9. - Publication Year :
- 1992
-
Abstract
- The molecular mechanism by which immunoglobulin (Ig) gene rearrangement occurs is highly conserved between mammalian and avian species. However, in avian species, an equivalent to the mammalian pre-B cell, which has undergone Ig heavy-chain gene rearrangement and expresses mu heavy chains in the absence of Ig light-chain rearrangement, has not been convincingly demonstrated. It is consequently unclear whether an ordered progression of gene rearrangement events leading to functional Ig expression occurs in avian species. To examine the sequence of Ig gene rearrangement events in chicken B-cell development, we transformed day 12 embryo bursal cells with the REV-T(CSV) retrovirus. More than 100 clones were analyzed by Southern blotting and polymerase chain reaction for the presence of Ig gene rearrangements. The majority of these clones contained only germline Ig sequences. Several clones contained complete heavy- and light-chain rearrangements and 13 clones contained only heavy-chain rearrangements analogous to stages of mammalian B-cell development. However, 5 clones contained rearrangements of light-chain genes in the absence of complete heavy-chain rearrangement. Consequently, we conclude that rearrangement of chicken Ig light-chain genes does not require heavy-chain variable-region rearrangement. This observation suggests that chicken Ig gene rearrangement events required for Ig expression occur stochastically rather than sequentially.
- Subjects :
- Alleles
Animals
Base Sequence
Blotting, Southern
Bursa of Fabricius embryology
Bursa of Fabricius immunology
Cell Transformation, Viral
Chick Embryo
Chickens
Cloning, Molecular
DNA genetics
DNA isolation & purification
Immunoglobulin Heavy Chains genetics
Molecular Sequence Data
Oligodeoxyribonucleotides
Polymerase Chain Reaction methods
Sequence Homology, Nucleic Acid
Stochastic Processes
B-Lymphocytes immunology
Gene Rearrangement
Genes, Immunoglobulin
Immunoglobulin Light Chains genetics
Immunoglobulin Variable Region genetics
Retroviridae genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0027-8424
- Volume :
- 89
- Issue :
- 16
- Database :
- MEDLINE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 1502173
- Full Text :
- https://doi.org/10.1073/pnas.89.16.7615