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Genetic characterisation of Cryptosporidium from a wild population of eastern grey kangaroos Macropus giganteus inhabiting a water catchment.
- Source :
-
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases [Infect Genet Evol] 2004 Mar; Vol. 4 (1), pp. 59-67. - Publication Year :
- 2004
-
Abstract
- Molecular characterisation of Cryptosporidium oocysts isolated from faeces collected from eastern grey kangaroos Macropus giganteus inhabiting an Australian water catchment revealed that this host was susceptible to three types of Cryptosporidium. Nucleotide sequence analysis of the 18S rDNA, Cryptosporidium oocyst wall protein (COWP) and a 70kDa heat shock protein (HSP70) identified an isolate identical to the described Cryptosporidium 'marsupial' genotype. A second isolate had less than 0.5% variation, compared to the described Cryptosporidium 'marsupial' genotype, within the sequences of the 18S rDNA, COWP and HSP70 and 10% variation in the internal transcribed spacer 1 (ITS1). Multilocus analysis of the third Cryptosporidium revealed a novel genotype that had a degree of genetic variation, at the four loci characterised, which was greater than or equivalent to that used to discriminate between currently recognised Cryptosporidium species. These findings have increased our current understanding on the molecular epidemiology of Cryptosporidium in Australian wildlife and have provided information on the types of Cryptosporidium marsupials may shed into the environment.
- Subjects :
- Animals
Base Sequence
Cryptosporidium classification
Feces parasitology
Genetic Variation
Genotype
HSP70 Heat-Shock Proteins genetics
Humans
Molecular Sequence Data
Phylogeny
Protozoan Proteins genetics
Sequence Alignment
Sequence Analysis, DNA
Cryptosporidiosis veterinary
Cryptosporidium genetics
Environment
Macropodidae parasitology
Subjects
Details
- Language :
- English
- ISSN :
- 1567-1348
- Volume :
- 4
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases
- Publication Type :
- Academic Journal
- Accession number :
- 15019591
- Full Text :
- https://doi.org/10.1016/j.meegid.2004.01.002