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In vivo detection of ribozyme cleavage products and RNA structure by use of terminal transferase-dependent PCR.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2004; Vol. 252, pp. 109-24. - Publication Year :
- 2004
-
Abstract
- Terminal transferase-dependent PCR (TDPCR) can be used after reverse transcription to analyze RNA. This method (RT-TDPCR) is able to provide in vivo information at nucleotide-level resolution, and has been used for study of ribozymes, RNA size, RNA structure, and RNA-protein interactions. A detailed protocol of RT-TDPCR is presented here with examples of its use in detecting ribozyme cleavage intermediates in yeast and a RNA transcription start site in mammalian cells.
- Subjects :
- Base Sequence
DNA Primers
Indicators and Reagents
Nucleic Acid Conformation
Polymerase Chain Reaction methods
RNA genetics
Reverse Transcriptase Polymerase Chain Reaction methods
Saccharomyces cerevisiae genetics
Transcription, Genetic
RNA chemistry
RNA metabolism
RNA, Catalytic chemistry
RNA, Catalytic metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1064-3745
- Volume :
- 252
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 15017045
- Full Text :
- https://doi.org/10.1385/1-59259-746-7:109