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Synergistic effect of hyperosmotic agents of dimethyl sulfoxide and glycerol on optical clearing of gastric tissue studied with near infrared spectroscopy.
- Source :
-
Physics in medicine and biology [Phys Med Biol] 2004 Feb 07; Vol. 49 (3), pp. 457-68. - Publication Year :
- 2004
-
Abstract
- In an effort to find an effective concentration that could minimize the side effect for clinical applications, and to understand the potential synergistic effect of hyperosmotic agents on optical clearing of gastric tissues, porcine stomach tissues (pyloric mucosa) applied with a mixed solution of glycerol and dimethyl sulfoxide (DMSO) are investigated with near infrared reflectance spectroscopy. Five chemical solutions, containing 80% glycerol, 50% DMSO, 50% glycerol with 10% DMSO, 20% DMSO and 30% DMSO, respectively, are prepared and studied; all of which show significant improvement in light transmittance, and thus reduction of the light scattering of tissue. It is found that, among the solutions investigated, 50% glycerol with 30% DMSO achieves the best clearing effect on the improvement of light penetration. Light transmittance is increased approximately 29% and diffuse reflectance decreased approximately 31% at 30 min after the topical application of 50% glycerol with 30% DMSO. This solution shows significantly stronger effect than 80% glycerol on optical clearing even though they have the same osmolarity. 80% glycerol leads to 23% increase of light transmittance and 24% decrease of diffuse reflectance. The mixed solution of 50% glycerol and 20% DMSO has less osmolarity than the solution of 80% glycerol, but they achieve a similar degree of optical clearing. In other words, the clearing effect of glycerol is enhanced by adding DMSO into it. It is suggested that membrane penetration and carrier effect of DMSO probably accounts for this synergistic effect.
- Subjects :
- Animals
Body Water drug effects
Body Water metabolism
Contrast Media
Culture Techniques methods
Dehydration chemically induced
Dehydration metabolism
Drug Synergism
Gastric Mucosa radiation effects
Light
Metabolic Clearance Rate
Osmosis
Scattering, Radiation
Staining and Labeling methods
Stomach drug effects
Stomach radiation effects
Swine
Dimethyl Sulfoxide pharmacology
Gastric Mucosa drug effects
Gastric Mucosa metabolism
Glycerol pharmacology
Spectrophotometry, Infrared methods
Tomography, Optical Coherence methods
Water metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0031-9155
- Volume :
- 49
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Physics in medicine and biology
- Publication Type :
- Academic Journal
- Accession number :
- 15012013
- Full Text :
- https://doi.org/10.1088/0031-9155/49/3/008