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Effect of C-reactive protein on chemokine expression in human aortic endothelial cells.
- Source :
-
Journal of molecular and cellular cardiology [J Mol Cell Cardiol] 2004 Mar; Vol. 36 (3), pp. 405-10. - Publication Year :
- 2004
-
Abstract
- Inflammation plays a pivotal role in atherosclerosis. In addition to being a risk marker for cardiovascular disease, much recent data support a role for C-reactive protein (CRP) in atherogenesis. Interleukin-8 (IL-8), a member of the CXC chemokines promotes monocyte-endothelial cell adhesion and arrest and is abundant in atherosclerotic plaques. However, there is a paucity of data examining the effect of CRP on IL-8 secretion in human aortic endothelial cells (HAEC). In this report, we show that incubation of HAEC with CRP resulted in a time and dose-dependent increase in IL-8 protein and mRNA via transcription. In contrast to human umbilical vein endothelial cells, monocyte-chemoattractant protein-1 expression in HAEC was not affected by CRP. Furthermore, CRP upregulated NF-kappa B activity in HAEC and inhibitors of NF-kappa B significantly reversed the upregulation of IL-8 by CRP. Blocking antibodies to IL-8 significantly decreased monocyte-endothelial cell adhesion induced by CRP (31%, P<0.01). In conclusion, this study makes the novel observation that CRP induces IL-8 synthesis and secretion in HAEC via upregulation of NF-kappa B activity.
- Subjects :
- Arteriosclerosis metabolism
Cell Adhesion drug effects
Cells, Cultured
Chemokine CCL2 genetics
Humans
Interleukin-8 genetics
NF-kappa B genetics
NF-kappa B metabolism
Peptides pharmacology
RNA, Messenger biosynthesis
RNA, Messenger genetics
Sesquiterpenes pharmacology
Aorta cytology
C-Reactive Protein pharmacology
Chemokine CCL2 biosynthesis
Endothelial Cells metabolism
Gene Expression Regulation drug effects
Interleukin-8 biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 0022-2828
- Volume :
- 36
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of molecular and cellular cardiology
- Publication Type :
- Academic Journal
- Accession number :
- 15010279
- Full Text :
- https://doi.org/10.1016/j.yjmcc.2003.12.005