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Identification of Ehrlichia chaffeensis morulae in cerebrospinal fluid mononuclear cells.
- Source :
-
Journal of clinical microbiology [J Clin Microbiol] 1992 Aug; Vol. 30 (8), pp. 2207-10. - Publication Year :
- 1992
-
Abstract
- We report a case of ehrlichiosis in a 72-year-old man who developed extreme lethargy, acute renal failure requiring hemodialysis, and respiratory insufficiency requiring intubation. Lumbar puncture performed on the second day of hospitalization revealed significant cellular pleocytosis. Ehrlichia morulae were tentatively identified in mononuclear cells in routinely processed Wright-stained cytospin preparations of cerebrospinal fluid (CSF). Identification was confirmed by a specific immunocytochemical staining procedure. Subsequent identification specifically as Ehrlichia chaffeensis morulae was established by polymerase chain reaction analysis, which revealed E. chaffeensis-specific DNA in CSF, bone marrow, and blood samples; by indirect fluorescent-antibody analysis, the patient developed an antibody titer of 32,768 against E. chaffeensis antigen. The patient responded to intravenous therapy with doxycycline and dexamethasone. Subsequently, neurologic, hematologic, renal, and pulmonary status had returned to baseline at follow-up 12 weeks after admission. To our knowledge, this is the first identification of E. chaffeensis morulae in CSF cells in an infected patient.
- Subjects :
- Aged
Bacteriological Techniques
DNA, Bacterial genetics
Ehrlichia classification
Ehrlichia genetics
Humans
Male
Polymerase Chain Reaction
Rickettsiaceae Infections cerebrospinal fluid
Rickettsiaceae Infections microbiology
Ehrlichia isolation & purification
Leukocytes, Mononuclear microbiology
Rickettsiaceae Infections diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 0095-1137
- Volume :
- 30
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Journal of clinical microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 1500537
- Full Text :
- https://doi.org/10.1128/jcm.30.8.2207-2210.1992